The inhibition of fungal contamination of food by natural plant essential oils is receiving widespread attention. In this study, 1 μL/mL -anisaldehyde showed remarkable antifungal ability against spore germination and mycelium growth. Experiments of peanut seeds infection showed that 4 μL/mL -anisaldehyde inhibited the development of spores on peanuts by 95.5% and the production of aflatoxin B1 production by 69.4%. Transcriptome analysis suggested that differentially expressed genes were primarily associated with spore growth, cell wall, cell membrane, oxidative stress, and energy metabolism. Physiological and biochemical experiments as well as scanning electron microscopy, fluorescence staining, and enzyme activity assay, further confirmed that -anisaldehyde destroyed the cell wall, permeability, and ergosterol biosynthesis of the cell membrane and led to decreased mitochondrial membrane potential and accumulated reactive oxygen species. This study provided new knowledge on the antifungal activity of -anisaldehyde and its mechanism of action, as well as theoretical support for -anisaldehyde as a possible antifungal agent for the control of contamination of food crops.

中文翻译：

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基于转录组分析的对茴香醛抗黄曲霉菌机制

天然植物精油抑制食品真菌污染正受到广泛关注。在本研究中，1 μL/mL 的茴香醛对孢子萌发和菌丝体生长表现出显着的抗真菌能力。花生种子侵染实验表明，4μL/mL的茴香醛可抑制花生孢子的发育95.5%，抑制黄曲霉毒素B1的产生69.4%。转录组分析表明差异表达基因主要与孢子生长、细胞壁、细胞膜、氧化应激和能量代谢相关。生理生化实验以及扫描电镜、荧光染色和酶活性测定进一步证实，β-茴香醛破坏细胞壁、通透性和细胞膜的麦角甾醇生物合成，导致线粒体膜电位下降和活性氧积累。 。该研究为β-茴香醛的抗真菌活性及其作用机制提供了新的认识，并为β-茴香醛作为可能的抗真菌剂用于控制粮食作物污染提供了理论支持。