Shao, S., Zhang, Y., Yin, K., Zhang, Y., Wei, L., & Wang, Q. (2022). FabR senses long-chain unsaturated fatty acids to control virulence in pathogen Edwardsiella piscicida. Molecular Microbiology, 117(4): 737–753. https://doi.org/10.1111/mmi.14869
In the above article, the authors compiled Figure 3n (the interaction between FabR and the promoter of cadB) incorrectly by inadvertently using the same image of the binding of FabR to the promoter of cfa (Figure 3b). The results in Figure 3 were used to validate ChIP-seq data with EMSA. The correct image for Figure 3n is shown below.
The authors sincerely apologize for this error and emphasize that they do not affect the conclusions reported in the paper.
FIGURE 3
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Validation of FabR regulon by EMSA, ChIP-qPCR, and qRT-PCR analyses. (a–p) Validation of the ChIP-seq results with EMSAs. The enrichment of each of the FabR-controlled promoters (i.e., cfa (a), fadD (c), glpT (e), sseA (g), lldP (i), ETAE_3163 (k), and cadB (m)) bound by FabR are shown in brackets. EMSAs were performed with purified FabR-MBP, 20 ng of each Cy5-labeled probe, and 20 ng of nonspecific competitor DNA poly(dI:dC). Shifts in the DNA probes were detected without (–) or with the addition of 100 and 200 nM of FabR-MBP protein. B: FabR-bound DNA; U: unbound DNA. The enrichment of FabR-binding at the gyrB (o and p) promoter was used as a control. (q) The relative enrichment of FabR binding to various promoters was analyzed by ChIP-qPCR. The results are presented as the mean ± SD. (n = 3). (r) qRT-PCR analysis of the transcripts of the indicated genes in ΔfabR to WT of cells grown in DMEM. *p < 0.05 (Student's t-test) compared with gyrB enrichment (q) or transcript level (r).