WLIP, WLIPβ, and WLIPγ Produced from Pseudomonas canadensis Q3–1 via Precursor-Directed Biosynthesis and Their Roles on Biocontrol of Phytophthora Blight in Peppers,Journal of Agricultural and Food Chemistry

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WLIP, WLIPβ, and WLIPγ Produced from Pseudomonas canadensis Q3–1 via Precursor-Directed Biosynthesis and Their Roles on Biocontrol of Phytophthora Blight in Peppers
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2024-02-16 , DOI: 10.1021/acs.jafc.3c07360
Zongwang Ma ₁ , Xiao Yang ₁ , Jun Sheng ₁

Affiliation

White line-inducing principle (WLIP, 1), together with two new cyclic lipopeptides (CLPs) WLIPβ (2) and WLIPγ (3), were characterized from the supernatant of Pseudomonas canadensis Q3–1 via precursor-directed biosynthesis (PDB) in the current study. They were purified from the supernatant of P. canadensis Q3–1 by solid-phase extraction (SPE) and high-performance liquid chromatography (HPLC), and their structures were mainly determined via bioinformatic analyses, spectrometric and spectroscopic techniques, as well as single crystal X-ray diffraction (XRD). These WLIPs share (R)-3-hydroxydecanoic acid (HDA), but they differ from each other in the composition of peptidic sequences. In addition, these CLPs showed biocontrol activities against Phytophthora blight (caused by Phytophthora capsici) in peppers. Collectively, this study has shown that PDB could be used for generating new CLPs in Pseudomonas spp. Moreover, we have confirmed that WLIP, WLIPβ, and WLIPγ could be used as lead agrochemicals to control Phytophthora blight in peppers.

中文翻译：

加拿大假单胞菌 Q3-1 通过前体定向生物合成产生的 WLIP、WLIPβ 和 WLIPγ 及其在辣椒疫病生物防治中的作用

白线诱导原理 (WLIP, 1 ) 以及两种新的环状脂肽 (CLP) WLIPβ ( 2 ) 和 WLIPγ ( 3 ) 通过前体定向生物合成 (PDB) 从加拿大假单胞菌Q3-1 的上清液中进行表征目前的研究。它们是通过固相萃取（SPE）和高效液相色谱（HPLC）从P. canadensis Q3-1的上清液中纯化出来的，其结构主要通过生物信息学分析、光谱分析和波谱技术以及单一方法确定。晶体X射线衍射(XRD)。这些 WLIP 共享 ( R )-3-羟基癸酸 (HDA)，但它们在肽序列的组成方面彼此不同。此外，这些 CLP 对辣椒中的疫霉病（由辣椒疫霉引起）表现出生物防治活性。总的来说，这项研究表明 PDB 可用于在假单胞菌属中生成新的 CLP。此外，我们已经证实WLIP、WLIPβ和WLIPγ可以用作铅农药来防治辣椒疫病。

更新日期：2024-02-16

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