This work aimed to investigate the protective effects of collagen hydrolysates containing different contents of Gly-Pro-Xaa tripeptides on UV-induced photoaging mice and to identify potent anti-photoaging peptides. Results showed that oral ingestion of collagen hydrolysates with a higher content of Gly-Pro-Xaa tripeptides (∼11.4%, HCH) dramatically enhanced the absorption of Pro-Hyp, Hyp-Gly, and Gly-Pro-Hyp into the body, which were 1.77-, 2.18-, and 65.07-fold higher in area under the concentration–time curve (AUC) values than that of collagen hydrolysates with a lower content of Gly-Pro-Xaa tripeptides (∼3.8%, LCH), respectively. Furthermore, the protective effects of HCH on the photo-aged skin of mice were significantly stronger than those of LCH in terms of increases in the contents of hyaluronic acid and collagen, improvement in skin elasticity and epidermal thickness, alleviation in inflammation, and decreases in the contents of matrix metalloproteinase-1 (MMP-1) and MMP-3. More importantly, Gly-Pro-Hyp displayed potent anti-photoaging activities comparable to HCH based on an equivalent amount of Hyp. Network pharmacology analysis for potential mechanisms further indicated that Gly-Pro-Hyp might interact with JUN and FOS and regulate IL-17 and TNF signaling pathways. Collectively, our results suggested that HCH had great potential to be applied in functional foods for skin health and Gly-Pro-Hyp was found to be a potent collagen-derived anti-photoaging peptide, which might contribute to the excellent anti-photoaging effects of HCH.

中文翻译：

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胶原蛋白水解物对紫外线诱导的光老化小鼠的影响：Gly-Pro-Hyp 作为有效的抗光老化肽

本工作旨在研究含有不同含量Gly-Pro-Xaa三肽的胶原蛋白水解物对紫外线诱导的光老化小鼠的保护作用，并鉴定有效的抗光老化肽。结果表明，口服摄入具有较高含量 Gly-Pro-Xaa 三肽（~11.4%，六氯环己烷）的胶原蛋白水解物可显着增强 Pro-Hyp、Hyp-Gly 和 Gly-Pro-Hyp 进入体内的吸收，浓度-时间曲线下面积 (AUC) 值分别比 Gly-Pro-Xaa 三肽含量较低 (~3.8%, LCH) 的胶原蛋白水解物高 1.77 倍、2.18 倍和 65.07 倍。此外，六氯环己烷对小鼠光老化皮肤的保护作用在增加透明质酸和胶原蛋白含量、改善皮肤弹性和表皮厚度、减轻炎症、减少炎症等方面均明显强于LCH。基质金属蛋白酶-1（MMP-1）和MMP-3的含量。更重要的是，Gly-Pro-Hyp 显示出与基于等量 Hyp 的 HCH 相当的有效抗光老化活性。潜在机制的网络药理学分析进一步表明，Gly-Pro-Hyp 可能与 JUN 和 FOS 相互作用，并调节 IL-17 和 TNF 信号通路。总的来说，我们的结果表明，六氯环己烷在皮肤健康功能性食品中具有巨大的应用潜力，而 Gly-Pro-Hyp 被发现是一种有效的胶原蛋白衍生的抗光老化肽，这可能有助于 HCH 优异的抗光老化效果。六六六。