The enteric nervous system (ENS) is a large and complex part of the peripheral nervous system, and it is vital for gut homeostasis. To study the ENS, different hyper- and hypo-innervated model systems have been developed. The NSE-Noggin mouse model was described as one of the few models with a higher enteric neuronal density in the colon. However, in our hands NSE-Noggin mice did not present with a hyperganglionic phenotype. NSE-Noggin mice were phenotyped based on fur appearance, genotyped and DNA sequenced to demonstrate transgene and intact NSE-Noggin-IRES-EGFP construct presence, and RNA expression of Noggin was shown to be upregulated. Positive EGFP staining in the plexus of NSE-Noggin mice also confirmed Noggin protein expression. Myenteric plexus preparations of the colon were examined to quantify both the overall density of enteric neurons and the proportions of enteric neurons expressing specific subtype markers. The total number of enteric neurons in the colonic myenteric plexus of transgenic mice did not differ significantly from wild types, nor did the proportion of calbindin, calretinin, or serotonin immunoreactive myenteric neurons. Possible reasons as to why the hyperinnervated phenotype could not be observed in contrast with original studies using this mouse model are discussed, including study design, influence of microbiota, and other environmental variables.

肠神经系统（ENS）是周围神经系统的一个庞大而复杂的部分，对于肠道稳态至关重要。为了研究 ENS，开发了不同的神经支配过度和神经支配不足的模型系统。 NSE-Noggin小鼠模型被描述为结肠中肠神经元密度较高的少数模型之一。然而，在我们手中，NSE-Noggin小鼠并未表现出超神经节表型。根据毛皮外观对NSE-Noggin小鼠进行表型分析、基因分型和 DNA 测序，以证明转基因和完整的 NSE-Noggin-IRES-EGFP 构建体的存在，并且Noggin的 RNA 表达被上调。 NSE-Noggin小鼠神经丛中的阳性 EGFP 染色也证实了 Noggin 蛋白的表达。检查结肠肌间丛制剂以量化肠神经元的总体密度和表达特定亚型标记物的肠神经元的比例。转基因小鼠结肠肌间神经丛中肠神经元的总数与野生型小鼠没有显着差异，钙结合蛋白、钙结合蛋白或血清素免疫反应性肌间神经元的比例也没有显着差异。讨论了与使用该小鼠模型的原始研究相比无法观察到过度神经支配表型的可能原因，包括研究设计、微生物群的影响和其他环境变量。