从海水中分离出三种革兰氏反应阳性细菌菌株，分别命名为 KSW-18 T、 KSW2-22 和 KSW4-11 T，并分别在韩国济州岛郭支海滩采集了两份干海藻样品，并对其进行了分析。通过多相方法检查分类位置。 16S rRNA基因系统发育表明，菌株KSW4-11 T与油酸微杆菌NBRC 103075 T密切相关，而菌株KSW-18 T和KSW2-22在包括上述两个菌株的进化枝的基础上形成了独特的亚系。这三种分离株彼此之间（99.7-99.9%；1-4 nt 差异）和油酸微杆菌（99.8-99.9%；1-3 nt 差异）显示出高度的序列相似性。化学分类学特征是微杆菌属的典型特征；赖氨酸作为肽聚糖的诊断二氨基酸和N-乙醇酰化胞壁酸，MK-11、MK-10 和 MK-12 的主要甲基萘醌，anteiso-C 15:0和 anteiso-C 17:0的主要脂肪酸，以及主要的极性脂质，包括二磷脂酰甘油、磷脂酰甘油和两种或三种未鉴定的糖脂。在基于核心基因组的系统发育树中，菌株KSW-18 T和KSW2-22与油酸微杆菌NBRC 103075 T密切相关，而菌株KSW4-11 T在包括上述三个菌株的进化枝的基础上形成了一个独特的亚系，与 16S rRNA 基因树对比。菌株KSW-18 T和KSW2-22彼此的OrthoANIu共有98.6%，数字DNA-DNA杂交为87.6%，表明它们是同一个物种的菌株，而菌株之间的OrthoANIu和数字DNA-DNA杂交值KSW-18 T和 KSW4-11 T以及这两个分离株与微杆菌属所有成员之间的差值分别≤86.5% 和≤30.7%。总体基因组相关性指数和表型差异性的分析支持这三个分离株代表微杆菌属的两个新物种。根据此处获得的结果，Microbacter aquilitoris sp。十一月（典型菌株 KSW-18 T = KCTC 49623 T = NBRC 115222 T）和Microbacter gwkjiense sp.十一月(类型菌株 KSW4-11 T = KACC 23321 T = DSM 116380 T ) 被提议。
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Microbacterium aquilitoris sp. nov. and Microbacterium gwkjiense sp. nov., isolated from beach
Three Gram-reaction-positive bacterial strains, designated KSW-18T, KSW2-22, and KSW4-11T, were isolated from seawater, and two dried seaweed samples collected at Gwakji Beach in Jeju, Republic of Korea, respectively, and their taxonomic positions were examined by a polyphasic approach. The 16S rRNA gene phylogeny showed that strain KSW4-11T was tightly associated with Microbacterium oleivorans NBRC 103075T, while strains KSW-18T and KSW2-22 formed a distinctive subline at the base of a clade including the above two strains. The three isolates showed high sequence similarity with one another (99.7–99.9%; 1–4 nt differences) and Microbacterium oleivorans (99.8–99.9%; 1–3 nt differences). The chemotaxonomic features were typical for the genus Microbacterium; Lysine as the diagnostic diamino acid and N-glycolylated muramic acid of the peptidoglycans, the predominant menaquinones of MK-11, MK-10 and MK-12, the major fatty acids of anteiso-C15:0 and anteiso-C17:0, and the major polar lipids including diphosphatidylglycerol, phosphatidylglycerol, and two or three unidentified glycolipids. In core genome-based phylogenetic tree, strains KSW-18T and KSW2-22 were closely associated with Microbacterium oleivorans NBRC 103075T, while strain KSW4-11T formed a distinctive subline at the base of a clade including the above three strains, in contrast to the 16S rRNA gene tree. Strains KSW-18T and KSW2-22 shared an OrthoANIu of 98.6% and a digital DNA–DNA hybridization of 87.6% with each other, representing that they were strains of a species, while the OrthoANIu and digital DNA–DNA hybridization values between strains KSW-18T and KSW4-11T, and between both of these isolates and all members of the genus Microbacterium were ≤86.5% and ≤30.7%, respectively. The analyses of overall genomic relatedness indices and phenotypic distinctness support that the three isolates represent two new species of the genus Microbacterium. Based on the results obtained here, Microbacterium aquilitoris sp. nov. (type strain KSW-18T = KCTC 49623T = NBRC 115222T) and Microbacterium gwkjiense sp. nov. (type strain KSW4-11T = KACC 23321T = DSM 116380T) are proposed.
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