D-glucuronic acid is a kind of glucose derivative, which has excellent properties such as anti-oxidation, treatment of liver disease and hyperlipidemia, and has been widely used in medicine, cosmetics, food and other fields. The traditional production methods of D-glucuronic acid mainly include natural extraction and chemical synthesis, which can no longer meet the growing market demand. The production of D-glucuronic acid by biocatalysis has become a promising alternative method because of its high efficiency and environmental friendliness. This review describes different production methods of D-glucuronic acid, including single enzyme catalysis, multi-enzyme cascade, whole cell catalysis and co-culture, as well as the intervention of some special catalysts. In addition, some feasible enzyme engineering strategies are provided, including the application of enzyme immobilized scaffold, enzyme mutation and high-throughput screening, which provide good ideas for the research of D-glucuronic acid biocatalysis.

D-葡萄糖醛酸是一种葡萄糖衍生物，具有抗氧化、治疗肝病、高血脂等优异性能，已广泛应用于医药、化妆品、食品等领域。 D-葡萄糖醛酸的传统生产方法主要包括天然提取和化学合成，已不能满足日益增长的市场需求。生物催化法生产D-葡萄糖醛酸因其高效、环境友好而成为一种有前景的替代方法。本文介绍了D-葡萄糖醛酸的不同生产方法，包括单酶催化、多酶级联、全细胞催化和共培养，以及一些特殊催化剂的干预。此外，还提供了一些可行的酶工程策略，包括酶固定支架的应用、酶突变和高通量筛选，为D-葡萄糖醛酸生物催化的研究提供了良好的思路。