Cell and Tissue Research ( IF 3.2 ) Pub Date : 2024-02-13 , DOI: 10.1007/s00441-024-03859-w
M Dieris 1 , D Kowatschew 1 , T Hassenklöver 2, 3 , I Manzini 2, 3 , S I Korsching 1
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The odor space of aquatic organisms is by necessity quite different from that of air-breathing animals. The recognized odor classes in teleost fish include amino acids, bile acids, reproductive hormones, nucleotides, and a limited number of polyamines. Conversely, a significant portion of the fish olfactory receptor repertoire is composed of trace amine-associated receptors, generally assumed to be responsible for detecting amines. Zebrafish possess over one hundred of these receptors, but the responses of olfactory sensory neurons to amines have not been known so far. Here we examined odor responses of zebrafish olfactory epithelial explants at the cellular level, employing calcium imaging. We report that amines elicit strong responses in olfactory sensory neurons, with a time course characteristically different from that of ATP-responsive (basal) cells. A quantitative analysis of the laminar height distribution shows amine-responsive cells undistinguishable from ciliated neurons positive for olfactory marker protein. This distribution is significantly different from those measured for microvillous neurons positive for transient receptor potential channel 2 and basal cells positive for proliferating cell nuclear antigen. Our results suggest amines as an important odor class for teleost fish.
中文翻译:
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成体嗅觉上皮的钙成像揭示胺是鱼类重要的气味类别
水生生物的气味空间必然与呼吸空气的动物有很大不同。硬骨鱼中公认的气味类别包括氨基酸、胆汁酸、生殖激素、核苷酸和有限数量的多胺。相反,鱼类嗅觉受体库的很大一部分是由痕量胺相关受体组成,通常被认为负责检测胺。斑马鱼拥有一百多个这样的受体,但嗅觉感觉神经元对胺的反应目前尚不清楚。在这里,我们利用钙成像在细胞水平上检查了斑马鱼嗅觉上皮外植体的气味反应。我们报告说,胺会在嗅觉感觉神经元中引起强烈反应,其时间过程与 ATP 反应(基底)细胞的反应不同。层流高度分布的定量分析显示胺反应细胞与嗅觉标记蛋白阳性的纤毛神经元无法区分。该分布与瞬时受体电位通道 2 呈阳性的微绒毛神经元和增殖细胞核抗原呈阳性的基底细胞的测量结果显着不同。我们的结果表明胺是硬骨鱼的重要气味类别。