<br>标准和模拟宿主介质中昆布多糖-金纳米颗粒的抗菌膜和抗毒活性,Applied Microbiology and Biotechnology

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标准和模拟宿主介质中昆布多糖-金纳米颗粒的抗菌膜和抗毒活性
Applied Microbiology and Biotechnology ( IF 3.9 ) Pub Date : 2024-02-13 , DOI: 10.1007/s00253-024-13050-4
Nazia Tabassum _{1,

2} , Fazlurrahman Khan _{1,

2,

3} , Geum-Jae Jeong ₄ , Dokyung Oh _{1,

2,

4} , Young-Mog Kim _{1,

2,

4}

Affiliation

抽象的

病原菌中迅速上升的抗菌素耐药性（AMR）已成为最严重的公共卫生挑战之一，死亡率很高。大多数病原菌已被认为是抗菌素耐药性的来源，也是由于生物膜的形成和毒力因子的产生而导致抗菌治疗失败的主要障碍。在这项工作中，纳米技术被用作控制生物膜形成并减弱铜绿假单胞菌和金黄色葡萄球菌毒力特征的替代方法。我们从海洋来源的昆布多糖中合成了生物相容性金纳米粒子，作为潜在的生物膜和毒力治疗方法。昆布多糖-金纳米粒子 (Lam-AuNPs) 已被鉴定为球形，尺寸为 49.84 ± 7.32 nm，zeta 电位为 − 26.49 ± 1.29 mV。在标准培养基和模拟宿主培养基中，Lam-AuNP 对几种耐药微生物病原体的 MIC 值在 2 至 1024 μg/mL 之间变化。据报道，Lam-AuNP 的亚 MIC 值可有效减少标准培养基和模拟宿主生长培养基中铜绿假单胞菌和金黄色葡萄球菌生物膜的产生。此外，Lam-AuNPs 的亚 MIC 强烈降低了铜绿假单胞菌中的溶血、绿脓素、pyoverdine、蛋白酶以及几种形式的鞭毛和菌毛介导的运动。 Lam-AuNPs 还抑制金黄色葡萄球菌溶血和淀粉样原纤维的产生。 Lam-AuNPs 以剂量依赖性方式强烈分散这些病原体预先形成的成熟生物膜。 Lam-AuNPs 将被认为是控制铜绿假单胞菌和金黄色葡萄球菌感染的替代抗生物膜和抗毒剂。

关键点

• 生物合成Lam-AuNP 以控制生物膜和毒力。

• Lam-AuNPs 在标准和模拟宿主介质中显示出有效的生物膜抑制作用。

• Lam-AuNPs 抑制铜绿假单胞菌和金黄色葡萄球菌的多种毒力因子。

"点击查看英文标题和摘要"

Antibiofilm and antivirulence activities of laminarin-gold nanoparticles in standard and host-mimicking media

Abstract

The rapidly rising antimicrobial resistance (AMR) in pathogenic bacteria has become one of the most serious public health challenges, with a high death rate. Most pathogenic bacteria have been recognized as a source of AMR and a primary barrier to antimicrobial treatment failure due to the development of biofilms and the production of virulence factors. In this work, nanotechnology was employed as a substitute method to control the formation of biofilms and attenuate virulence features in Pseudomonas aeruginosa and Staphylococcus aureus. We synthesized biocompatible gold nanoparticles from marine-derived laminarin as potential biofilm and virulence treatments. Laminarin-gold nanoparticles (Lam-AuNPs) have been identified as spherical, 49.84 ± 7.32 nm in size and − 26.49 ± 1.29 mV zeta potential. The MIC value of Lam-AuNPs against several drug-resistant microbial pathogens varied from 2 to 1024 μg/mL in both standard and host-mimicking media. Sub-MIC values of Lam-AuNPs were reported to effectively reduce the production of P. aeruginosa and S. aureus biofilms in both standard and host-mimicking growth media. Furthermore, the sub-MIC of Lam-AuNPs strongly reduced hemolysis, pyocyanin, pyoverdine, protease, and several forms of flagellar and pili-mediated motility in P. aeruginosa. Lam-AuNPs also inhibited S. aureus hemolysis and the production of amyloid fibrils. The Lam-AuNPs strongly dispersed the preformed mature biofilm of these pathogens in a dose-dependent manner. The Lam-AuNPs would be considered an alternative antibiofilm and antivirulence agent to control P. aeruginosa and S. aureus infections.