Integrated Nondestructive Spectroscopic Technology to Reveal the Influence Mechanism of Lignins from Pretreated Corn Stover on Cellulose Saccharification,ACS Sustainable Chemistry & Engineering

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Integrated Nondestructive Spectroscopic Technology to Reveal the Influence Mechanism of Lignins from Pretreated Corn Stover on Cellulose Saccharification
ACS Sustainable Chemistry & Engineering ( IF 7.1 ) Pub Date : 2024-02-08 , DOI: 10.1021/acssuschemeng.3c07981
Xiaoxue Zhao ₁ , Jiahao Wang ₁ , Kai Lan ₂ , Zhichen Zhao ₁ , Chenhuan Lai ₁ , Caoxing Huang ₁ , Qiang Yong ₁

Affiliation

The changes in the structure of lignin during the pretreatment processes of biomass can affect its subsequent enzymatic hydrolysis efficiency. To explore the influence mechanism of dilute acid and hydrothermal pretreatment of corn stover lignin on cellulose saccharification, an integrated nondestructive spectral technology with fluorescence spectroscopy, surface plasmon resonance, and atomic force microscopy was performed. It showed that the surface lignins on the corn stover with dilute acid pretreatment at 190 °C (DA190-SL) and hydrothermal pretreatment at 190 °C (HP190-SL) possessed enhancement (from 76.95 to 80.09%) and inhibition (from 76.95 to 61.91%) for the enzymatic digestibility of Avicel, respectively. Nondestructive spectroscopic analysis indicated that HP190-SL adsorbed onto cellulase is mainly driven by hydrogen bonding and van der Waals forces, while the DA190-SL-enzyme system was mainly driven by hydrophobic interactions. The association affinity of DA190-SL combined with cellulase was higher than that of HP190-SL. The binding force of the HP190-SL-enzyme (0.16 nN) is lower than that of the DA190-SL-enzyme (0.75 nN), which leads to a higher propensity for dissociation of HP190-SL from cellulase after binding. This study aims to establish a theoretical basis for regulating the enzymatic performance during the hydrothermal and dilute acid pretreatment of corn stover at the molecular level.

中文翻译：

集成无损光谱技术揭示预处理玉米秸秆木质素对纤维素糖化的影响机制

生物质预处理过程中木质素结构的变化会影响其后续的酶解效率。为了探究玉米秸秆木质素稀酸和水热预处理对纤维素糖化的影响机制，采用荧光光谱、表面等离子体共振和原子力显微镜集成无损光谱技术。结果表明，经过190 ℃稀酸预处理（DA190-SL）和190 ℃水热预处理（HP190-SL）的玉米秸秆表面木质素具有增强作用（从76.95％到80.09％）和抑制作用（从76.95％到80.09％）。 61.91%）分别为 Avicel 的酶消化率。无损光谱分析表明HP190-SL吸附在纤维素酶上主要是通过氢键和范德华力驱动的，而DA190-SL-酶系统主要是通过疏水相互作用驱动的。DA190-SL与纤维素酶结合的亲和力高于HP190-SL。HP190-SL-酶的结合力(0.16 nN)低于DA190-SL-酶的结合力(0.75 nN)，这导致结合后HP190-SL与纤维素酶解离的倾向更高。本研究旨在为玉米秸秆水热和稀酸预处理过程中酶性能的分子水平调控奠定理论基础。

更新日期：2024-02-08

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