Controlled initiation of biochemical events and in particular of protein activity is a powerful tool in biochemical research. Specifically, optical trigger signals are an attractive approach for remote control of enzyme activity. We present a method for generating optical control of enzyme activity applicable to a widespread range of enzymes. The approach is based on short laser pulses as optical "switches" introducing an instantaneous change of the pH-value for activation of protein function. The pH-jump is induced by proton release from 2-nitrobenzaldehyde. Reaction conditions were chosen to yield a pH-jump of almost 3 units on switching from inactive to active conditions for the enzyme. In this experimental setup, irradiation can be realized without any loss of enzyme activity. Following this change in pH-value, a controlled activation of hydrolytic activity of acid phosphatase is successfully demonstrated. This application provides a general method for photocontrol of enzymatic function for proteins having a significant pH-profile. The kinetic data for the substrate 6-chloro-8-fluoro-4-methylumbelliferone phosphate are determined.

中文翻译：

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激光诱导 pH 跳跃对酶活性的光开关

生化事件特别是蛋白质活性的受控启动是生化研究中的有力工具。具体而言，光触发信号是远程控制酶活性的一种有吸引力的方法。我们提出了一种用于产生适用于广泛酶的酶活性的光学控制的方法。该方法基于短激光脉冲作为光学“开关”，引入 pH 值的瞬时变化以激活蛋白质功能。pH 值跳跃是由 2-硝基苯甲醛释放质子引起的。选择反应条件以在酶从非活性条件切换到活性条件时产生几乎 3 个单位的 pH 跃变。在这个实验装置中，可以在不损失酶活性的情况下实现辐照。随着 pH 值的这种变化，成功地证明了酸性磷酸酶水解活性的受控激活。该应用提供了一种光控制具有显着 pH 曲线的蛋白质的酶功能的通用方法。测定底物 6-chloro-8-fluoro-4-methylumbelliferone 磷酸盐的动力学数据。