A novel yeast-mediated hydrogenation was developed for the synthesis of neohesperidin dihydrochalcone (NHDC) in high yields (over 83%). Moreover, whole-cell catalytic hydrolysis was also designed to hydrolyze NHDC into potential sweeteners, hesperetin dihydrochalcone-7-O-glucoside (HDC-G) and hesperetin dihydrochalcone (HDC). The biohydrogenation was further combined with whole-cell hydrolysis to achieve a one-pot two-step biosynthesis, utilizing yeast to hydrogenate C═C in the structure, while Aspergillus niger cells hydrolyze glycosides. The conversion of NHDC and the proportion of hydrolysis products could be controlled by adjusting the catalysts, the components of the reaction system, and the addition of glucose. Furthermore, yeast-mediated biotransformation demonstrated superior reaction stability and enhanced safety and employed more cost-effective catalysts compared to the traditional chemical hydrogenation of NHDC synthesis. This research not only provides a new route for NHDC production but also offers a safe and flexible one-pot cascade biosynthetic platform for the production of high-value compounds from citrus processing wastes.

中文翻译：

---

新橙皮苷二氢查耳酮的微生物驱动合成和水解：生物转化过程和可行性研究

开发了一种新型酵母介导的氢化反应，用于高产率（超过 83%）合成新橙皮苷二氢查尔酮 (NHDC)。此外，全细胞催化水解还被设计用于将NHDC水解成潜在的甜味剂，橙皮素二氢查尔酮-7- O-葡萄糖苷（HDC-G）和橙皮素二氢查尔酮（HDC）。生物氢化进一步与全细胞水解相结合，实现一锅两步生物合成，利用酵母氢化结构中的C=C，而黑曲霉细胞水解糖苷。NHDC的转化率和水解产物的比例可以通过调节催化剂、反应体系的组成以及葡萄糖的添加量来控制。此外，与NHDC合成的传统化学氢化相比，酵母介导的生物转化表现出优异的反应稳定性和更高的安全性，并采用更具成本效益的催化剂。该研究不仅为NHDC生产提供了一条新途径，而且为利用柑橘加工废弃物生产高价值化合物提供了安全、灵活的一锅级联生物合成平台。