To date, many potent compounds have been found which are derived from plants and herbs and possess anticancer properties due to their antioxidant effects. 9″-Lithospermic acid methyl ester is an effective natural compound derived from the Thymus thracicus Velen. It has been proven that this compound has substantial properties in different diseases, but its effects in cancer have not been thoroughly evaluated. The aim of this work was to study the effects of 9″-Lithospermic acid methyl ester (9″-methyl lithospermate) in U87 and T98 glioblastoma cell lines. Its effects on cellular viability were assessed via Trypan Blue and Crystal Violet stains, the cell cycle analysis through flow cytometry, and cell migration by employing the scratch wound healing assay. The results demonstrated that 9″-methyl lithospermate was able to inhibit cellular proliferation, induce cellular death, and inhibit cell migration. Furthermore, these results were intensified by the addition of temozolomide, the most prominent chemotherapeutic drug in glioblastoma tumors. Further studies are needed to reproduce these findings in animal models and investigate if 9″-lithospermic acid methyl ester represents a potential new therapeutic addition for gliomas.

中文翻译：

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9″-紫精酸甲酯对胶质母细胞瘤细胞的抗肿瘤活性


迄今为止，已发现许多有效的化合物，这些化合物源自植物和草药，并且由于其抗氧化作用而具有抗癌特性。 9'-Lithospermicacidmethylester 是一种有效的天然化合物，源自 Thymus thracicus Velen。已证明这种化合物在不同疾病中具有显着的特性，但其对癌症的作用尚未得到彻底评估。这项工作的目的是研究 9”-紫精酸甲酯（9”-紫精酸甲酯）对 U87 和 T98 胶质母细胞瘤细胞系的影响。通过台盼蓝和结晶紫染色评估其对细胞活力的影响，通过流式细胞术进行细胞周期分析，并通过划痕伤口愈合测定进行细胞迁移。结果表明，9'-甲基紫精酸盐能够抑制细胞增殖、诱导细胞死亡并抑制细胞迁移。此外，通过添加替莫唑胺（胶质母细胞瘤中最重要的化疗药物）强化了这些结果。需要进一步的研究来在动物模型中重现这些发现，并调查 9'-紫精酸甲酯是否代表神经胶质瘤的潜在新治疗添加物。