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Biodecolorization and biotransformation of methylene blue using mixed cultures of brown-rot fungus Daedalea dickinsii and filamentous fungus Aspergillus oryzae: identification of metabolites and degradation pathway
RSC Advances ( IF 3.9 ) Pub Date : 2024-02-08 , DOI: 10.1039/d3ra08544a Adi Setyo Purnomo 1 , Umirul Solichah Fauzany 1 , Hamdan Dwi Rizqi 1 , Taufiq Rinda Alkas 2 , Ichiro Kamei 3
RSC Advances ( IF 3.9 ) Pub Date : 2024-02-08 , DOI: 10.1039/d3ra08544a Adi Setyo Purnomo 1 , Umirul Solichah Fauzany 1 , Hamdan Dwi Rizqi 1 , Taufiq Rinda Alkas 2 , Ichiro Kamei 3
Affiliation
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This study aimed to examine biodecolorization and biotransformation of methylene blue (MB) using mixed cultures of brown-rot fungus Daedalea dickinsii and filamentous fungus Aspergillus oryzae. In addition, the ratio of D. dickinsii and A. oryzae in mixed cultures was 1 : 1, and the sample was incubated at 30 °C for 7 days in liquid medium potato dextrose broth (PDB). The results showed that the sample had the ability to remove and transform 95.24 mg L−1 MB. In this study, mixed cultures had the highest removal percentage of 64.77%, while values of 5.94% and 36.82% were obtained for single cultures of D. dickinsii and A. oryzae, respectively. LC-TOF/MS analysis results showed that peak intensity of MB compound (m/z 284) in each treatment chromatogram decreased compared to the control. The metabolites of decolorization by D. dickinsii were C15H16N3S, C16H19N3SO, and C16H21N3SO, while C31H48N3S+ was obtained using A. oryzae. For mixed cultures, the metabolites obtained included C26H37N2O3S, C9H8N2O3S, C28H38NO2S, and C27H27N5S2. Based on the results, mixed cultures of D. dickinsii and A. oryzae had a high MB decolorization and could be used in the textile industry.
中文翻译：
利用褐腐真菌 Daedalea dickinsii 和丝状真菌米曲霉混合培养物对亚甲蓝进行生物脱色和生物转化：代谢物鉴定和降解途径
本研究旨在利用褐腐真菌Daedalea dickinsii和丝状真菌米曲霉的混合培养物检测亚甲蓝 (MB) 的生物脱色和生物转化。此外，混合培养物中狄更斯菌和米曲霉的比例为1:1，并且将样品在液体培养基马铃薯葡萄糖肉汤(PDB)中于30℃培养7天。结果表明，样品具有去除转化95.24 mg L -1 MB的能力。在本研究中，混合培养物的去除率最高，为 64.77%，而单一培养物狄更斯菌和米曲霉的去除率分别为 5.94% 和 36.82%。 LC-TOF/MS分析结果表明，与对照相比，各处理色谱图中MB化合物的峰强度( m / z 284)有所降低。狄更斯菌脱色的代谢产物为C 15 H 16 N 3 S、C 16 H 19 N 3 SO、C 16 H 21 N 3 SO，而米曲霉脱色代谢产物为C 31 H 48 N 3 S + 。 对于混合培养物，获得的代谢物包括C 26 H 37 N 2 O 3 S、C 9 H 8 N 2 O 3 S、C 28 H 38 NO 2 S和C 27 H 27 N 5 S 2 。结果表明，狄更斯菌和米曲霉的混合培养物具有较高的MB脱色率，可用于纺织工业。
更新日期：2024-02-08
中文翻译：
利用褐腐真菌 Daedalea dickinsii 和丝状真菌米曲霉混合培养物对亚甲蓝进行生物脱色和生物转化：代谢物鉴定和降解途径
本研究旨在利用褐腐真菌Daedalea dickinsii和丝状真菌米曲霉的混合培养物检测亚甲蓝 (MB) 的生物脱色和生物转化。此外，混合培养物中狄更斯菌和米曲霉的比例为1:1，并且将样品在液体培养基马铃薯葡萄糖肉汤(PDB)中于30℃培养7天。结果表明，样品具有去除转化95.24 mg L -1 MB的能力。在本研究中，混合培养物的去除率最高，为 64.77%，而单一培养物狄更斯菌和米曲霉的去除率分别为 5.94% 和 36.82%。 LC-TOF/MS分析结果表明，与对照相比，各处理色谱图中MB化合物的峰强度( m / z 284)有所降低。狄更斯菌脱色的代谢产物为C 15 H 16 N 3 S、C 16 H 19 N 3 SO、C 16 H 21 N 3 SO，而米曲霉脱色代谢产物为C 31 H 48 N 3 S + 。 对于混合培养物，获得的代谢物包括C 26 H 37 N 2 O 3 S、C 9 H 8 N 2 O 3 S、C 28 H 38 NO 2 S和C 27 H 27 N 5 S 2 。结果表明，狄更斯菌和米曲霉的混合培养物具有较高的MB脱色率，可用于纺织工业。
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