The current climate change situation could bring critical effects for marine species, especially those already considered endangered. Although some species can adapt fast to the environmental changes, it is necessary to get into the worst scenario and develop tools to anticipatedly assess the physiological effects of such environmental change. With this purpose, our study aims to determine the effect of a range of seawater temperatures and pHs on sperm motility in the European eel (Anguilla anguilla). Low seawater pH (6.5–7.4) decreased the eel sperm motility in comparison to the control (pH = 8.2). We also studied the combined effect of the pH of the artificial seminal plasma (the plasma where the sperm cells are suspended) with the pH of Artificial Sea Water (ASW, pH 7.8 or and 8.2). We did not find statistical differences in sperm motility and kinetic parameters caused by the artificial seminal plasma pH. However, seawater pH induced significantly higher values of total sperm motility, and the percentage of fast spermatozoa with a pH of 8.2 in comparison with a pH of 7.8. In contrast, the seawater temperature did not affect sperm motility parameters or sperm longevity. To study the effect of the interaction between seawater temperature and pH on sperm motility, two temperatures: 4 and 24 °C, and two pHs 7.8 and 8.2, were tested. There were significant differences between temperature and pH in several kinetic parameters and, in general, the lowest values were observed in the samples activated at low temperature and low pH (4 °C, pH 7.8). This work suggest that eel sperm motility and kinetics will not be affected by the expected changes in pH or temperature due to the climate change.

当前的气候变化形势可能会给海洋物种带来严重影响，特别是那些已经被认为濒临灭绝的物种。尽管有些物种可以快速适应环境变化，但有必要进入最坏的情况并开发工具来预期评估这种环境变化的生理影响。为此，我们的研究旨在确定一系列海水温度和 pH 值对欧洲鳗鱼 ( Anguilla anguilla ) 精子活力的影响。与对照（pH = 8.2）相比，低海水 pH 值（6.5–7.4）降低了鳗鱼精子的活力。我们还研究了人工精浆（精子细胞悬浮的血浆）的 pH 值与人工海水的 pH 值（ASW，pH 7.8 或 8.2）的综合影响。我们没有发现人工精浆pH值引起的精子活力和动力学参数的统计差异。然而，与 pH 值 7.8 相比，海水 pH 值导致总精子活力和快速精子百分比在 pH 值为 8.2 时显着升高。相比之下，海水温度不会影响精子活力参数或精子寿命。为了研究海水温度和 pH 值之间的相互作用对精子活力的影响，测试了两种温度：4 和 24 °C，以及两种 pH 值 7.8 和 8.2。温度和 pH 值之间的几个动力学参数存在显着差异，一般来说，在低温和低 pH 值（4 °C，pH 7.8）下激活的样品中观察到最低值。这项工作表明，鳗鱼精子的活力和动力学不会受到气候变化导致的 pH 值或温度的预期变化的影响。