Phenytoin drug was frequently prescribed to treat seizures and breast cancer, but chemically synthesized phenytoin seems to have additional adverse effects reported previously. Therefore, we have developed a phenytoin drug from Catharanthus roseus (CR) plants in nanoparticle (NPs) form for the first time using the photon-induced method (PIM). GC–MS analysis of CR-NPs revealed all the peaks indicating the presence of a phenytoin compound, and 1H NMR analysis confirmed the chemical shift of the phenytoin structure. The PXRD pattern indicates a highly crystalline nature, with a crystallite size of 25 nm. The IC50 value for the inhibition of cell growth by nano-sized particles of Catharanthus roseus (CR-NPs) is 15.91 μg/mL, as observed in MCF-7 cell lines. Furthermore, the fabricated CR-NPs electrode exhibited a specific capacitance of 62 Fg−1 and a current density of 1 Ag−1, with a cyclic retention of 89.27 % even after 2000 cycles. Our research offers a new perspective on the advancements of nano-sized plants, which have a significant impact on the development of natural drugs for anticancer treatment and electrochemical analysis.

中文翻译：

---

评估长春花植物苯妥英纳米颗粒的抗菌和电化学性能


苯妥英药物经常被用来治疗癫痫和乳腺癌，但化学合成的苯妥英似乎有先前报道的额外副作用。因此，我们首次利用光子诱导方法（PIM）从长春花（CR）植物中开发出纳米颗粒（NP）形式的苯妥英药物。 CR-NP 的 GC-MS 分析显示所有峰表明存在苯妥英化合物，1H NMR 分析证实了苯妥英结构的化学位移。 PXRD 图案显示出高度结晶性质，微晶尺寸为 25 nm。在 MCF-7 细胞系中观察到，长春花纳米颗粒 (CR-NP) 抑制细胞生长的 IC50 值为 15.91 μg/mL。此外，所制备的CR-NPs电极的比电容为62 Fg−1，电流密度为1 Ag−1，即使在2000次循环后，循环保留率仍为89.27%。我们的研究为纳米植物的进步提供了新的视角，这对抗癌治疗和电化学分析天然药物的开发产生了重大影响。