Odd-numbered fatty acids (FAs) have been widely used in nutrition, agriculture, and chemical industries. Recently, some studies showed that they could be produced from bacteria or yeast, but the products are almost exclusively odd-numbered long-chain FAs. Here we report the design and construction of two biosynthetic pathways in for production of odd-numbered medium-chain fatty acids (OMFAs) via ricinoleic acid and 10-hydroxystearic acid, respectively. The production of OMFAs was enabled by introducing a hydroxy fatty acid cleavage pathway, including an alcohol dehydrogenase from , a Baeyer-Villiger monooxygenase from , and a lipase from . These OMFA biosynthetic pathways were optimized by eliminating the rate-limiting step, generating heptanoic acid, 11-hydroxyundec-9-enoic acid, nonanoic acid, and 9-hydroxynonanoic acid at 7.83 mg/L, 9.68 mg/L, 9.43 mg/L and 13.48 mg/L, respectively. This work demonstrates the biological production of OMFAs in a sustainable manner in .

中文翻译：

---

用于从头生产奇数中链脂肪酸的酿酒酵母代谢工程

奇数脂肪酸（FA）已广泛应用于营养、农业和化学工业。最近，一些研究表明它们可以由细菌或酵母生产，但产品几乎都是奇数长链 FA。在这里，我们报告了分别通过蓖麻油酸和10-羟基硬脂酸生产奇数中链脂肪酸（OMFA）的两种生物合成途径的设计和构建。OMFA 的生产是通过引入羟基脂肪酸裂解途径实现的，包括来自 的醇脱氢酶、来自 的 Baeyer-Villiger 单加氧酶和来自 的脂肪酶。这些 OMFA 生物合成途径通过消除限速步骤进行了优化，生成了 7.83 mg/L、9.68 mg/L、9.43 mg/L 的庚酸、11-羟基十一烯酸、壬酸和 9-羟基壬酸和 13.48 毫克/升，分别。这项工作展示了 OMFA 以可持续的方式进行生物生产。