Cells provide dynamic platforms for executing exogenous genetic programs in synthetic biology, resulting in highly context-dependent circuit performance. Recent years have seen an increasing interest in understanding the intricacies of circuit–host relationships, their influence on the synthetic bioengineering workflow, and in devising strategies to alleviate undesired effects. We provide an overview of how emerging circuit–host interactions, such as growth feedback and resource competition, impact both deterministic and stochastic circuit behaviors. We also emphasize control strategies for mitigating these unwanted effects. This review summarizes the latest advances and the current state of host-aware and resource-aware design of synthetic gene circuits.

中文翻译：

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鲁棒合成基因电路的上下文相关重新设计


细胞提供了在合成生物学中执行外源遗传程序的动态平台，从而产生高度依赖于环境的电路性能。近年来，人们越来越有兴趣了解复杂的电路-宿主关系、它们对合成生物工程工作流程的影响，以及设计减轻不良影响的策略。我们概述了新兴的电路-宿主相互作用（例如增长反馈和资源竞争）如何影响确定性和随机电路行为。我们还强调减轻这些不良影响的控制策略。这篇综述总结了合成基因电路的宿主感知和资源感知设计的最新进展和现状。