Microcarrier expansion systems show exciting potential to revolutionise mesenchymal stromal cell (MSC)-based clinical therapies by providing an opportunity for economical large-scale expansion of donor- and patient-derived cells. The poor reproducibility and efficiency of cell expansion on commercial polystyrene microcarriers have driven the development of novel microcarriers with tuneable physical, mechanical, and cell-instructive properties. These new microcarriers show innovation toward improving cell expansion outcomes, although their limited biological characterisation and compatibility with dynamic culture systems suggest the need to realign the microcarrier design pathway. Clear headway has been made toward developing infrastructure necessary for scaling up these technologies; however, key challenges remain in characterising the wholistic effects of microcarrier properties on the biological fate and function of expanded MSCs.

中文翻译：

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绘制现代化临床间充质基质细胞扩增的微载体设计途径


微载体扩增系统通过提供经济地大规模扩增供体和患者来源的细胞的机会，显示出彻底改变基于间充质基质细胞（MSC）的临床疗法的令人兴奋的潜力。商业聚苯乙烯微载体上细胞扩增的再现性和效率较差，推动了具有可调节的物理、机械和细胞指导特性的新型微载体的开发。这些新的微载体在改善细胞扩增结果方面表现出创新，尽管它们有限的生物学特性和与动态培养系统的兼容性表明需要重新调整微载体设计途径。在开发扩大这些技术所需的基础设施方面已经取得了明显进展；然而，在表征微载体特性对扩增的间充质干细胞的生物命运和功能的整体影响方面仍然存在关键挑战。