Temperature-Controlled Screening of Catechol Groups in Poly(N-Isopropylacrylamide-co-Dopamine Methacrylamide) for Cell Detachment,ACS Applied Polymer Materials

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Temperature-Controlled Screening of Catechol Groups in Poly(N-Isopropylacrylamide-co-Dopamine Methacrylamide) for Cell Detachment
ACS Applied Polymer Materials ( IF 4.4 ) Pub Date : 2024-02-01 , DOI: 10.1021/acsapm.3c01971
Liuxin Yang ₁ , Pengfei Ren ₁ , Dandan Wei ₁ , Min Liang ₁ , Li Xu ₁ , Yinghua Tao ₁ , Guanhua Jiao ₁ , Tianzhu Zhang ₁ , Qianli Zhang ₂

Affiliation

Temperature-responsive poly(N-isopropylacrylamide) (PNIPAM) polymers have been studied in many fields due to their “on–off” adjustable properties under temperature stimulation. In this work, the “on–off” mechanism for poly(N-isopropylacrylamide-co-dopamine methacrylamide) (PNIPAM-co-DMA) microgels for cell culture and nonenzymatic cell harvest was explored. Thermoresponsive surfaces were developed on silica wafers using microgels with different DMA contents using a simple spin-coating method. Experiments showed that the microgels showed an “on” state above the lower critical solution temperature (LCST), and PNIPAM-co-DMA (PND) microgels shrank to expose catechol groups for enhanced cell adhesion and proliferation. Conversely, the microgels showed an “off” state below LCST, and the hydrophobic and cell-repellent properties of DMA contributed to the rapid hydration of microgels for accelerated cell disassociation. The developed microgels presented DMA content and distribution-related cell detachment efficiency, with PND (100:0.5) and PND (100:2) showing better results. The failure of cell harvest caused by too much DMA restricted the shrinkage and swelling of microgels due to the strong hydrogen-bonding adhesion of catechol moieties for PND (100:10). Overall, the presented strategy exhibits a wide-ranging utility to construct thermoresponsive surfaces with “on–off” functionalization by introducing catechol chemical monomers.

中文翻译：

用于细胞分离的聚（N-异丙基丙烯酰胺-共-多巴胺甲基丙烯酰胺）中儿茶酚基团的温度控制筛选

温度响应型聚（N-异丙基丙烯酰胺）（PNIPAM）聚合物由于其在温度刺激下具有“开-关”可调特性，已在许多领域得到研究。在这项工作中，探索了用于细胞培养和非酶促细胞收获的聚（ N-异丙基丙烯酰胺-共-多巴胺甲基丙烯酰胺）（PNIPAM-共-DMA）微凝胶的“开关”机制。通过简单的旋涂方法，使用具有不同 DMA 含量的微凝胶在二氧化硅晶片上开发了热响应表面。实验表明，微凝胶在高于较低临界溶液温度（LCST）时显示出“开启”状态，并且 PNIPAM- co -DMA（PND）微凝胶收缩以暴露儿茶酚基团，从而增强细胞粘附和增殖。相反，微凝胶在低于 LCST 时显示出“关闭”状态，DMA 的疏水性和细胞排斥特性有助于微凝胶的快速水合，从而加速细胞解离。开发的微凝胶呈现出 DMA 含量和分布相关的细胞脱离效率，其中 PND (100:0.5) 和 PND (100:2) 显示出更好的结果。由于邻苯二酚部分对 PND (100:10) 具有很强的氢键粘附作用，过多 DMA 会导致细胞收获失败，从而限制了微凝胶的收缩和膨胀。总体而言，所提出的策略在通过引入儿茶酚化学单体构建具有“开关”功能化的热响应表面方面表现出广泛的实用性。

更新日期：2024-02-01

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