具有 POD 样和 GSH 样活性的含铜和铁的磷钨酸盐纳米颗粒可靶向抑制革兰氏阴性菌,ACS Applied Nano Materials

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具有 POD 样和 GSH 样活性的含铜和铁的磷钨酸盐纳米颗粒可靶向抑制革兰氏阴性菌
ACS Applied Nano Materials ( IF 5.3 ) Pub Date : 2024-02-03 , DOI: 10.1021/acsanm.3c05558
Yijing Zhang ₁ , Dezhi Yang _{1,

2} , Yaling Yang ₁

Affiliation

现阶段，生产强效抗菌药物来解决传统抗生素过度使用引起的多重耐药问题非常重要，因为抗生素的滥用对公众健康构成严重威胁。在此，报道了一种使用含铜和铁的磷钨酸盐（Cu，Fe-PT）靶向杀死革兰氏阴性菌的有效策略。Cu,Fe-PTs作为纳米酶可以通过氧化消耗谷胱甘肽，与少量H ₂ O ₂结合产生活性氧(ROS)，并靶向革兰氏阴性菌。Cu,Fe-PTs可以通过产生活性氧（ROS），即超氧自由基（^· O ₂^–）和羟基自由基（^· OH）来加速谷胱甘肽（GSH）的消耗。通过zeta电位和动力学参数可以证明，Cu,Fe-PTs的选择性抑制作用是由于Cu,Fe-PTs与革兰氏阴性菌结合后对H ₂ O ₂具有良好的亲和力，而稳定性纳米酶的性能也得到改善。此外，Cu,Fe-PTs通过Fe ²⁺ /Fe ³⁺和Cu ²⁺ /Cu ⁺之间的价态跃迁提供更多的活性位点，从而促进电子转移。有趣的是，研究结果表明，Cu,Fe-PTs可以靶向抑制革兰氏阴性菌，对大肠杆菌（E. coli）（∼99.4%）和耐氨苄青霉素大肠杆菌（AREC）（AREC）具有有效的抗菌特性。～99.9%）。这项研究为高效纳米酶的设计及其在靶向抗菌治疗领域的应用提供了策略。

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Cu- and Fe-Containing Phosphotungstate Nanoparticles with POD-like and GSH-like Activities to Target Inhibition of Gram-Negative Bacteria

At this stage, it is very important to produce strong antibacterial drugs to solve the problem of multidrug resistance caused by overuse of traditional antibiotics because the abuse of antibiotics poses a serious threat to public health. Herein, an effective strategy for targeted killing of Gram-negative bacteria using copper- and iron-containing phosphotungstates (Cu,Fe-PTs) has been reported. Cu,Fe-PTs as a nanozyme can consume glutathione through oxidation, combine with a small amount of H₂O₂ to produce reactive oxygen species (ROS), and target Gram-negative bacteria. Cu,Fe-PTs could accelerate glutathione (GSH) consumption through the generation of reactive oxygen species (ROS), namely superoxide radical (^•O₂^–) and hydroxyl radical (^•OH). It can be proved by the zeta potential and the kinetic parameters that the selective inhibition of Cu,Fe-PTs is due to the good affinity of Cu,Fe-PTs for H₂O₂ after binding to Gram-negative bacteria, while the stability of the nanozyme is also improved. In addition, Cu,Fe-PTs facilitate electron transfer by providing more active sites through valence transitions between Fe²⁺/Fe³⁺ and Cu²⁺/Cu⁺. Interestingly, the results of the study showed that Cu,Fe-PTs can be targeted to inhibit Gram-negative bacteria with effective antimicrobial properties against Escherichia coli (E. coli) (∼99.4%) and ampicillin-resistant Escherichia coli (AREC) (∼99.9%). This study provides a strategy for the design of efficient nanozymes and their applications in the field of targeted antimicrobial therapy.

更新日期：2024-02-03

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