Diabetes is a common metabolic disease worldwide, which often has serious complications and seriously threatens human health. Abnormal viscosity and peroxynitrite (ONOO) concentration are closely related to diabetes. Herein, we report a fluorescent dual response probe , which can simultaneously detect the changes of viscosity and ONOO in diabetes at dual fluorescence channels. sensitively responds to viscosity and ONOO with significant emission signal changes at 710 nm and 645 nm, respectively. Moreover, selectively targets mitochondria and generates a new compound when detecting ONOO, which can spontaneously target and light up another type of organelle (lipid droplets), thus showing spatially separated dual-channel fluorescence signals. More notably, was successfully used to simultaneously detect the viscosity and ONOO variations in hyperglycemic cells, diabetes and its complications (using mice models) for the first time, which may lead to a better understanding of the physiological and pathological phenomena of diabetes.

中文翻译：

---

双响应荧光探针同时检测糖尿病中的过氧亚硝酸盐和粘度

糖尿病是世界范围内常见的代谢性疾病，常出现严重并发症，严重威胁人类健康。粘度和过氧亚硝酸盐（ONOO）浓度异常与糖尿病密切相关。在此，我们报道了一种荧光双响应探针，它可以在双荧光通道上同时检测糖尿病中粘度和ONOO的变化。对粘度和 ONOO 做出敏感响应，分别在 710 nm 和 645 nm 处产生显着的发射信号变化。此外，在检测ONOO时选择性地靶向线粒体并生成新的化合物，它可以自发地靶向并点亮另一种类型的细胞器（脂滴），从而显示空间分离的双通道荧光信号。更值得注意的是，首次成功用于同时检测高血糖细胞、糖尿病及其并发症（使用小鼠模型）的粘度和ONOO变化，这可能有助于更好地了解糖尿病的生理和病理现象。