A Programmable DNAzyme for the Sensitive Detection of Nucleic Acids,Angewandte Chemie International Edition

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A Programmable DNAzyme for the Sensitive Detection of Nucleic Acids
Angewandte Chemie International Edition ( IF 16.1 ) Pub Date : 2024-01-30 , DOI: 10.1002/anie.202320179
Chenzhi Shi ₁ , Donglei Yang ₁ , Xiaowei Ma ₁ , Li Pan ₁ , Yuanchuan Shao ₂ , Gaurav Arya ₃ , Yonggang Ke ₄ , Chuan Zhang ₅ , Fuan Wang ₆ , Xiaolei Zuo ₁ , Min Li ₁ , Pengfei Wang ₁

Affiliation

A unimolecular self-locked allosteric DNAzyme biosensor (named SPOT) is developed to enable sensitive detection (LOD: fM-aM) of low-abundance nucleic acids in a one-pot, one-step, preamplification-free, isothermal detection manner. SPOT exhibits potent performance on miRNA-based detection for cancer diagnostics and viral RNA-based detection for SARS-CoV-2 infection diagnostics.

中文翻译：

用于灵敏检测核酸的可编程脱氧核糖核酸酶

开发了一种单分子自锁变构DNAzyme生物传感器（命名为SPOT），以一锅、一步、免预扩增、等温检测方式实现低丰度核酸的灵敏检测（LOD：fM-aM）。 SPOT 在基于 miRNA 的癌症诊断检测和基于病毒 RNA 的 SARS-CoV-2 感染诊断检测方面表现出强大的性能。

更新日期：2024-01-30

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