Abstract

The sodium salt of 7-ethylthio-3-nitro-1,2,4-triazolo[5,1-c]-1,2,4-triazin-4-one dihydrate (1) is one of promising antiviral compounds that have shown a pronounced biological activity against influenza virus subtype H5N1, West Nile, and SARS (severe acute respiratory syndrome). Using cyclic voltammetry, it was shown that the electrochemical activity of compound 1 in a Britton–Robinson buffer solution (BRB) of pH 2.0 on a glassy carbon electrode is due to the electrochemical reduction of the nitro group conjugated with the heterocyclic system. A method was developed for the determination of compound 1 by direct cathodic square-wave voltammetry. The linearity region of the calibration curve in a BRB solution of pH 7.0 is 10–300 mg/L (R² = 0.999), the limit of detection is 1.5 mg/L, and the limit of quantification is 4.5 mg/L. The accuracy of the developed procedure is close to 100%, the relative standard deviation is 3.6%.

中文翻译：

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伏安法测定潜在抗病毒药物 7-乙硫基-3-硝基-1,2,4-三唑并[5,1-c]-1,2,4-三嗪-4-一二水合钠盐

摘要

7-乙硫基-3-硝基-1,2,4-三唑并[5,1- c ]-1,2,4-三嗪-4-酮二水合物的钠盐 ( 1 ) 是一种有前景的抗病毒化合物，具有对流感病毒 H5N1 亚型、西尼罗河病毒和 SARS（严重急性呼吸系统综合症）表现出显着的生物活性。使用循环伏安法表明，化合物1在pH 2.0的Britton-Robinson缓冲溶液（BRB）中在玻碳电极上的电化学活性是由于与杂环体系共轭的硝基的电化学还原所致。建立了直接阴极方波伏安法测定化合物1的方法。pH 7.0 的 BRB 溶液中的校准曲线线性范围为 10–300 mg/L ( R ² = 0.999)，检测限为 1.5 mg/L，定量限为 4.5 mg/L。所开发程序的准确度接近100%，相对标准偏差为3.6%。