基于金纳米晶体和靶标诱导DNA循环信号放大的水果和蔬菜中氟虫腈的电化学传感,Sensors and Actuators B: Chemical

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基于金纳米晶体和靶标诱导DNA循环信号放大的水果和蔬菜中氟虫腈的电化学传感
Sensors and Actuators B: Chemical ( IF 8.0 ) Pub Date : 2024-01-30 , DOI: 10.1016/j.snb.2024.135420
Yiqing Wang , Ruiyi Li , Yirui Shen , Zaijun Li , Yishu Yan , Jing Yang

开发可检测水果和蔬菜中氟虫腈的高性能电化学适体传感器对于改善人类健康具有重要意义。本文提出了一种使用 21 肽作为形状诱导剂合成类金 (RC-Au) 纳米晶体的方法。合成过程涉及纳米晶体的同时生长及其不对称发展，从而形成RC-Au纳米颗粒，并增加其高折射率面的暴露，从而增强其催化活性。然后将 RC-Au 纳米晶体与适体/辅助 DNA 混合体结合，制造电化学适体传感器，该传感器可以通过释放游离辅助 DNA 链来检测氟虫腈，从而触发目标诱导的 DNA 循环。研究发现，一种氟虫腈分子可将许多 RC-Au-硫堇探针转移到适体传感器表面，从而导致探针的氧化和还原，从而导致信号放大。将氟虫腈的浓度从 1 × 10 M 增加到 1 × 10 M 会导致安培电流线性增加。检测限为 3.7 × 10 M (S/N = 3)。所开发的传感器比其他电化学氟虫腈传感器表现出更高的灵敏度，并且令人满意地用于检测水果和蔬菜中的氟虫腈。

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Electrochemical sensing of fipronil in fruit and vegetable based on gold nanocrystal and target-induced DNA cycle signal amplification

Development of a high-performance electrochemical aptasensor that can detect fipronil in fruits and vegetables is important for human health improvement. This paper presents a method for synthesising –like gold (RC-Au) nano-crystals using a type of 21 peptide as a shape inducer. The synthesis process involves the concurrent growth of nano-crystals and their asymmetric development, resulting in the formation of RC-Au nano-particles with increased exposure of their high-index facets, which enhances their catalytic activity. The RC-Au nano-crystals were then combined with an aptamer/auxiliary DNA hybrid to fabricate an electrochemical aptasensor that can sense fipronil via the release of the free auxiliary DNA strand, triggering target-induced DNA cycling. One fipronil molecule was found to transfer many RC-Au–thionine probes to the aptasensor surface, with the resulting oxidation and reduction of the probes leading to signal amplification. Increasing the concentration of fipronil from 1 × 10 to 1 × 10 M led to a linear increase in the amperometric current. A limit of detection of 3.7 × 10 M (S/N = 3) was obtained. The developed sensor exhibits higher sensitivity than other electrochemical fipronil sensors and was satisfactorily used to detect fipronil in fruits and vegetables.

更新日期：2024-01-30

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