<br>生理生化反应和转录组分析揭示磺胺甲恶唑对蛋白核小球藻固碳功能的影响和机制,Science of the Total Environment

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生理生化反应和转录组分析揭示磺胺甲恶唑对蛋白核小球藻固碳功能的影响和机制
Science of the Total Environment ( IF 8.2 ) Pub Date : 2024-01-28 , DOI: 10.1016/j.scitotenv.2024.170460
Yuhao Zhou ₁ , Yujiao Yue ₂ , Xinyang Chen ₂ , Feifan Wu ₂ , Wei Li ₂ , Pingping Li ₂ , Jiangang Han ₃

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磺胺甲恶唑（SMX）的发生具有低浓度、假持续性的特点。然而，SMX的毒性作用和机制，特别是低浓度和长期暴露，目前尚不清楚。本研究探讨了SMX在群体、生理生化和转录水平上对碳固定相关生物过程的影响和机制。结果表明，1~1000 μg/L SMX在21 d期间显着抑制干重和固碳率。超氧化物歧化酶 (SOD) 和过氧化氢酶 (CAT) 活性的上调以及丙二醛 (MDA) 的积累表明 SMX 对 . SMX 抑制碳酸酐酶 (CA) 的活性，从而刺激 Rubisco 的活性。主成分分析（PCA）显示，SMX浓度与Rubisco和CAT呈正相关，而暴露时间与CA呈负相关。转录分析表明，通过调节原卟啉IX的转移和叶绿素循环来稳定叶绿素-a的合成。同时，响应Rubisco对CO的需求，触发了多种CO补偿机制，包括光呼吸、类C CO补偿和嘌呤代谢途径。本研究为SMX生态风险的综合评估提供科学依据。

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Physiological-biochemical responses and transcriptomic analysis reveal the effects and mechanisms of sulfamethoxazole on the carbon fixation function of Chlorella pyrenoidosa

The occurrence of sulfamethoxazole (SMX) is characterized by low concentration and pseudo-persistence. However, the toxic effects and mechanisms of SMX, especially for low concentration and long-term exposure, are still not clear. This study investigated the effects and mechanisms of SMX on carbon fixation-related biological processes of at population, physiological-biochemical, and transcriptional levels. Results showed that 1–1000 μg/L SMX significantly inhibited the dry weight and carbon fixation rate of during 21 d. The upregulation of superoxide dismutase (SOD) and catalase (CAT) activities, as well as the accumulation of malondialdehyde (MDA) demonstrated that SMX posed oxidative damage to . SMX inhibited the activity of carbonic anhydrase (CA), and consequently stimulated the activity of Rubisco. Principal component analysis (PCA) revealed that SMX concentration was positively correlated with Rubisco and CAT while exposure time was negatively correlated with CA. Transcriptional analysis showed that the synthesis of chlorophyll-a was stabilized by regulating the diversion of protoporphyrin IX and the chlorophyll cycle. Meanwhile, multiple CO compensation mechanisms, including photorespiratory, C-like CO compensation and purine metabolism pathways were triggered in response to the CO requirements of Rubisco. This study provides a scientific basis for the comprehensive assessment of the ecological risk of SMX.

更新日期：2024-01-28

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