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Genomics of alphabaculovirus isolates infecting Mamestra species from North America and Eurasia
Journal of Invertebrate Pathology ( IF 3.6 ) Pub Date : 2024-01-28 , DOI: 10.1016/j.jip.2024.108063
Martin Erlandson 1 , Doug Baldwin 1 , Just M Vlak 2 , David Theilmann 3
Affiliation  

Whole genome sequencing and multiplex PCR analysis were used to characterize previously isolated baculovirus isolates from populations in Eurasia. Although these viruses have been previously described as Mamestra brassicae nucleopolyhedrovirus (MbNPV/MabrNPV), we demonstrate here that these isolates represent strains of the baculovirus species (MacoNPV-A) and (). The MabrNPV-Bu and –Uk isolates had 96% nucleotide (nt) identity to the type isolate MacoNPV-A 90/2 at the whole genome level and in addition contained a homologue which is found in MacoNPV-A but not MacoNPV-B. MabrNPV-Si, -De and –Nl had 96.6, 96.6 and 98.5% nt identity to the type isolate MacoNPV-B 96/2 at the whole genome level, respectively and contained a homologue. Gene content, synteny and K-2-P , and analysis also confirmed the presence of both MacoNPV-A and MacoNPV-B isolates in Eurasia. Thus, both these alphabaculovirus species have wide Holarctic distributions in host species. MacoNPV-A and MacoNPV-B have wide host ranges and in addition we showed that MacoNPV-B isolates trended to higher infectivity for larvae.

中文翻译:


感染北美和欧亚大陆 Mamestra 物种的甲杆病毒分离株的基因组学



使用全基因组测序和多重 PCR 分析来表征先前从欧亚大陆人群中分离出的杆状病毒分离株。尽管这些病毒之前被描述为甘蓝马梅斯特拉核多角体病毒 (MbNPV/MabrNPV),但我们在此证明这些分离株代表杆状病毒种 (MacoNPV-A) 和 ()。 MabrNPV-Bu 和 –Uk 分离株在全基因组水平上与分离株 MacoNPV-A 90/2 具有 96% 的核苷酸 (nt) 同一性,此外还含有在 MacoNPV-A 中发现的同源物,但在 MacoNPV-B 中没有发现。 MabrNPV-Si、-De和-Nl在全基因组水平上分别与分离株MacoNPV-B 96/2具有96.6%、96.6%和98.5%的nt同一性,并且含有同源物。基因含量、同线性和 K-2-P 以及分析也证实了欧亚大陆存在 MacoNPV-A 和 MacoNPV-B 分离株。因此,这两种甲杆病毒在宿主物种中具有广泛的全北分布。 MacoNPV-A 和 MacoNPV-B 具有广泛的宿主范围,此外我们还发现 MacoNPV-B 分离株对幼虫具有更高的感染性。
更新日期:2024-01-28
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