The model plant Physcomitrium patens has played a pivotal role in enhancing our comprehension of plant evolution and development. However, the current genome harbours numerous regions that remain unfinished and erroneous. To address these issues, we generated an assembly using Oxford Nanopore reads and Hi-C mapping. The assembly incorporates telomeric and centromeric regions, thereby establishing it as a near telomere-to-telomere genome except a region in chromosome 1 that is not fully assembled due to its highly repetitive nature. This near telomere-to-telomere genome resolves the chromosome number at 26 and provides a gap-free genome assembly as well as updated gene models to aid future studies using this model organism.

模式植物小立碗藓在增强我们对植物进化和发育的理解方面发挥了关键作用。然而，当前的基因组包含许多尚未完成和错误的区域。为了解决这些问题，我们使用 Oxford Nanopore 读取和 Hi-C 映射生成了一个组件。该组装包含端粒和着丝粒区域，从而将其建立为接近端粒到端粒的基因组，但 1 号染色体中的一个区域除外，该区域由于其高度重复性而未完全组装。这种接近端粒到端粒的基因组解析了 26 号染色体编号，并提供了无间隙基因组组装以及更新的基因模型，以帮助使用该模型生物体进行未来的研究。