The Odorant Receptor Recognizing Camphor in a Camphor Tree Specialist Orthaga achatina (Lepidoptera: Pyralidae),Journal of Agricultural and Food Chemistry

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The Odorant Receptor Recognizing Camphor in a Camphor Tree Specialist Orthaga achatina (Lepidoptera: Pyralidae)
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2024-01-24 , DOI: 10.1021/acs.jafc.3c08877
Yu Ma ₁ , Ting-Ting Yang ₁ , Shuang Ni ₁ , Ji-Xiang Wang ₁ , Yu He ₁ , Yu-Xiao Si ₁ , Jin Zhang ₁ , Shuang-Lin Dong ₁ , Qi Yan ₁

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Camphor has been used as an effective repellent and pesticide to stored products for a long history, but Orthaga achatina (Lepidoptera: Pyralidae) has evolved to specifically feed on the camphor tree Cinnamomum camphora. However, the behavioral response of O. achatina to camphor and the molecular basis of camphor perception are totally unknown. Here, we demonstrated that both male and female adults were behaviorally attracted to camphor, suggesting the adaptation of O. achatina to and utilization of camphor as a signal of C. camphora. Second, in 40 O. achatina OR genes obtained by analyzing antenna transcriptomes, only OachOR16/Orco significantly responded to camphor in the Xenopus oocyte system. Finally, by molecular docking analysis and site-directed mutagenesis, the Ser209 residue is confirmed to be essential for binding of the oachOR16 with camphor. This study not only reveals the camphor-based host plant choice and olfactory mechanisms of O. achatina but also provides a molecular target for screening more potential insect repellents.

中文翻译：

樟脑树中的气味受体识别樟脑专家 Orthaga achatina（鳞翅目：螟科）

长期以来，樟脑一直被用作储存产品的有效驱避剂和杀虫剂，但Orthaga achatina （鳞翅目：Pyralidae）已进化为专门以樟树Cinnamomum樟树为食。然而， O. achatina对樟脑的行为反应和樟脑感知的分子基础完全未知。在这里，我们证明雄性和雌性成虫在行为上都被樟脑吸引，这表明O. achatina适应并利用樟脑作为C. 樟脑的信号。其次，通过分析天线转录组获得的 40 个O. achatina OR基因中，只有 OachOR16/Orco 对非洲爪蟾卵母细胞系统中的樟脑有显着反应。最后，通过分子对接分析和定点诱变，证实Ser209残基对于oachOR16与樟脑的结合是必需的。该研究不仅揭示了O. achatina基于樟脑的寄主植物选择和嗅觉机制，而且为筛选更多潜在驱虫剂提供了分子靶点。

更新日期：2024-01-24

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