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Dynamic changes of lipids in Eucommia ulmoides rubber particles and the relationship between the biosynthesis of SQDG and E. ulmoides rubber
Journal of Rubber Research ( IF 1.2 ) Pub Date : 2024-01-24 , DOI: 10.1007/s42464-023-00232-4
Chuhan Zhang , Ting Yuan , Lijun Qin

Eucommia ulmoides rubber particles (EuRPs) are organelles that synthesise and store E. ulmoides rubber (EuR). In this study, the lipids dynamic change of EuRPs from leaf tissue at different growth period were investigated. The results showed that there were 1149 lipid species belonging to 39 lipid classes that were identified; TGs, DGs and Cers were the most abundant among all lipids of EuRPs. Lipid expression analysis demonstrated that the expression intensity of TGs was almost 1 × 104-fold and 10-fold higher than those of MGs and DGs, respectively. Among sphingolipids, the expression intensity of SoP, CerG2, GM3, SM and GerG3 increased throughout the duration of investigation (August–November 2021). Further analysis found that the Cers species accounted for 9.40% of total lipid species and the number of Cers species in EuRPs isolated from leaves in August was the most, with gradual decline in the following 3 months. Meanwhile, hierarchical clustering indicated that there were seven types of highly abundant lipid species in leaf EuRPs from September to November, i.e. SQDG (18:3/18:3), SQDG (34:3), SQDG (17:5/23:2), DGDG (38:12), Co (Q9), TG (16:0/16:0/18:3) and TG (39:1). Compared with leaf EuRPs in August, there were 79, 101 and 107 lipid species which showed significant differential expression in September, October and November. Based on the high expression intensity of SQDG in EuRPs, the correlation between the genes mediated in EuR or SQDG biosynthesis was further analysed, whereby the results indicated co-expression of some genes, which implies the synergistic regulatory role in SQDG EuR biosynthesis.



中文翻译:

杜仲橡胶颗粒脂质动态变化及SQDG与杜仲橡胶生物合成的关系

杜仲 橡胶颗粒(EuRPs)是合成和储存杜仲橡胶(EuR)的细胞器。本研究研究了不同生育期叶片组织中EuRPs的脂质动态变化。结果显示,已鉴定出属于 39 个脂质类别的 1149 种脂质; EuRPs 的所有脂质中,TG、DG 和 Cers 含量最高。脂质表达分析表明,TGs的表达强度分别比MGs和DGs高近1×10 4倍和10倍。在鞘脂中,SoP、CerG2、GM3、SM 和 GerG3 的表达强度在整个研究期间(2021 年 8 月至 11 月)不断增加。进一步分析发现,Cers种类占总脂质种类的9.40%,且8月份从叶片中分离得到的EuRPs中Cers种类数量最多,随后3个月逐渐下降。同时,层次聚类表明9-11月叶片EuRPs中存在7种高丰度脂质种类,即 SQDG (18:3/18:3)、SQDG (34:3)、SQDG (17:5/23:2)、DGDG (38:12)、Co (Q9)、TG (16:0/16:0) /18:3) 和 TG (39:1)。与8月叶片EuRPs相比,9月、10月和11月出现显着差异表达的脂质种类分别为79、101和107个。基于SQDG在EuRPs中的高表达强度,进一步分析了EuR或SQDG生物合成中介导的基因之间的相关性,结果表明一些基因是共表达的,这意味着SQDG在EuR生物合成中具有协同调节作用。

更新日期:2024-01-24
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