<br>立碗藓和被孢霉内生菌之间的多层次分析探讨了陆地植物和真菌之间潜在的长期相互作用,The Plant Journal

当前位置： X-MOL 学术 › Plant J. › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

立碗藓和被孢霉内生菌之间的多层次分析探讨了陆地植物和真菌之间潜在的长期相互作用
The Plant Journal ( IF 6.2 ) Pub Date : 2024-01-24 , DOI: 10.1111/tpj.16605
Davis Mathieu _{1,

2} , Abigail E Bryson _{1,

2} , Britta Hamberger ₂ , Vasanth Singan ₃ , Keykhosrow Keymanesh ₃ , Mei Wang ₃ , Kerrie Barry ₃ , Stephen Mondo _{3,

4,

5} , Jasmyn Pangilinan ₃ , Maxim Koriabine ₃ , Igor V Grigoriev _{3,

6} , Gregory Bonito _{1,

7} , Björn Hamberger _{1,

2}

Affiliation

模式苔藓物种小立碗藓（Physcomitrium patens）长期以来一直被用于研究从苔藓植物到被子植物的陆地植物的分化。除了系统发育关系之外，有限数量的差异组织以及与最早的有胚植物相似的形态提供了一个代表基本植物结构的系统。根据当今植物与真菌的相互作用，推测这些王国之间存在着长期的关系，早于植物陆化化。被孢霉科的起源不同于其他陆地真菌，与苔藓植物的分化相似，与丛枝菌根真菌有关，但它们是自由生活的，观察到与植物相互作用，并且可以在全球苔藓微生物组中找到。由于它们的平行起源，我们在这里评估两种被孢霉科物种， Linnemannia elongata和Benniella erionia ，如何与共培养中的P. patens相互作用。我们还评估了这些真菌的Mollicute相关或Burkholderia相关的细菌内共生体（MRE 或 BRE）如何影响植物反应。通过高通量表型组学、显微镜检查、RNA 测序、差异表达分析、基因本体富集以及 99 个其他P. patens转录组研究之间的比较来研究共培养相互作用。在这里，我们提出了新的高通量方法来测量P. patens生长，识别传统琼脂培养基上未表达的 800 多个基因的新表达，识别P. patens和被孢霉科之间的微妙相互作用，并观察植物-真菌相互作用依赖的变化是否存在 MRE 或 BRE。我们的研究根据今天观察到的植物和真菌伙伴之间的通讯，以及将L. elongata和B. erionia鉴定为与P. patens一起的现代真菌内生菌，提供了对植物和真菌伙伴如何相互作用的见解。

"点击查看英文标题和摘要"

Multilevel analysis between Physcomitrium patens and Mortierellaceae endophytes explores potential long-standing interaction among land plants and fungi

The model moss species Physcomitrium patens has long been used for studying divergence of land plants spanning from bryophytes to angiosperms. In addition to its phylogenetic relationships, the limited number of differential tissues, and comparable morphology to the earliest embryophytes provide a system to represent basic plant architecture. Based on plant–fungal interactions today, it is hypothesized these kingdoms have a long-standing relationship, predating plant terrestrialization. Mortierellaceae have origins diverging from other land fungi paralleling bryophyte divergence, are related to arbuscular mycorrhizal fungi but are free-living, observed to interact with plants, and can be found in moss microbiomes globally. Due to their parallel origins, we assess here how two Mortierellaceae species, Linnemannia elongata and Benniella erionia, interact with P. patens in coculture. We also assess how Mollicute-related or Burkholderia-related endobacterial symbionts (MRE or BRE) of these fungi impact plant response. Coculture interactions are investigated through high-throughput phenomics, microscopy, RNA-sequencing, differential expression profiling, gene ontology enrichment, and comparisons among 99 other P. patens transcriptomic studies. Here we present new high-throughput approaches for measuring P. patens growth, identify novel expression of over 800 genes that are not expressed on traditional agar media, identify subtle interactions between P. patens and Mortierellaceae, and observe changes to plant–fungal interactions dependent on whether MRE or BRE are present. Our study provides insights into how plants and fungal partners may have interacted based on their communications observed today as well as identifying L. elongata and B. erionia as modern fungal endophytes with P. patens.

更新日期：2024-01-24

点击分享查看原文

点击收藏

阅读更多本刊新发论文