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Metabolic Remodulation of Chassis and Corn Stover Bioprocessing to Unlock 3-Hydroxypropionic Acid Biosynthesis from Agrowaste-Derived Substrates
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2024-01-23 , DOI: 10.1021/acs.jafc.3c08419 Lei Zhou 1 , Yufei Zhang 1 , Tingting Chen 1 , Junhua Yun 2 , Mei Zhao 1 , Hossain M Zabed 2 , Cunsheng Zhang 1 , Xianghui Qi 1, 2
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2024-01-23 , DOI: 10.1021/acs.jafc.3c08419 Lei Zhou 1 , Yufei Zhang 1 , Tingting Chen 1 , Junhua Yun 2 , Mei Zhao 1 , Hossain M Zabed 2 , Cunsheng Zhang 1 , Xianghui Qi 1, 2
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Embracing the principles of sustainable development, the valorization of agrowastes into value-added chemicals has nowadays received significant attention worldwide. Herein, Escherichia coli was metabolically rewired to convert cellulosic hydrolysate of corn stover into a key platform chemical, namely, 3-hydroxypropionic acid (3-HP). First, the heterologous pathways were introduced into E. coli by coexpressing glycerol-3-P dehydrogenase and glycerol-3-P phosphatase in both single and fusion (gpdp12) forms, making the strain capable of synthesizing glycerol from glucose. Subsequently, a glycerol dehydratase (DhaB123-gdrAB) and an aldehyde dehydrogenase (GabD4) were overexpressed to convert glycerol into 3-HP. A fine-tuning between glycerol synthesis and its conversion into 3-HP was successfully established by 5′-untranslated region engineering of gpdp12 and dhaB123-gdrAB. The strain was further metabolically modulated to successfully prevent glycerol flux outside the cell and into the central metabolism. The finally remodulated chassis produced 32.91 g/L 3-HP from the cellulosic hydrolysate of stover during fed-batch fermentation.
中文翻译：
底盘和玉米秸秆生物加工的代谢重新调节，以从农作物废弃物衍生的基质中解锁 3-羟基丙酸的生物合成
秉承可持续发展的原则，将农业废弃物转化为高附加值化学品如今已受到全世界的广泛关注。在此，大肠杆菌进行了代谢重组，将玉米秸秆的纤维素水解产物转化为关键的平台化学品，即 3-羟基丙酸 (3-HP)。首先，通过以单一形式和融合形式（ gpdp 12）共表达甘油-3-P脱氢酶和甘油-3-P磷酸酶（gpdp 12）将异源途径引入大肠杆菌，使该菌株能够从葡萄糖合成甘油。随后，甘油脱水酶（DhaB123-gdrAB）和醛脱氢酶（GabD4）被过表达，将甘油转化为3-HP。通过gpdp 12 和dhaB 123- gdrAB的 5'-非翻译区工程成功地建立了甘油合成及其转化为 3-HP 之间的微调。该菌株经过进一步代谢调节，成功阻止甘油流出细胞并进入中央代谢。最终重新调节的底盘在补料分批发酵过程中从秸秆的纤维素水解产物中产生了 32.91 g/L 3-HP。
更新日期：2024-01-23
中文翻译：
底盘和玉米秸秆生物加工的代谢重新调节，以从农作物废弃物衍生的基质中解锁 3-羟基丙酸的生物合成
秉承可持续发展的原则，将农业废弃物转化为高附加值化学品如今已受到全世界的广泛关注。在此，大肠杆菌进行了代谢重组，将玉米秸秆的纤维素水解产物转化为关键的平台化学品，即 3-羟基丙酸 (3-HP)。首先，通过以单一形式和融合形式（ gpdp 12）共表达甘油-3-P脱氢酶和甘油-3-P磷酸酶（gpdp 12）将异源途径引入大肠杆菌，使该菌株能够从葡萄糖合成甘油。随后，甘油脱水酶（DhaB123-gdrAB）和醛脱氢酶（GabD4）被过表达，将甘油转化为3-HP。通过gpdp 12 和dhaB 123- gdrAB的 5'-非翻译区工程成功地建立了甘油合成及其转化为 3-HP 之间的微调。该菌株经过进一步代谢调节，成功阻止甘油流出细胞并进入中央代谢。最终重新调节的底盘在补料分批发酵过程中从秸秆的纤维素水解产物中产生了 32.91 g/L 3-HP。
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