Hectorite is trioctahedral clay mineral that possess unique characteristics of surface reactivity and adsorption, cation exchange capacity and easy delamination in water into individual nano layers. Thus, hectorite can be a promising nanocarrier with biocompatibility, high payload and controlled release of drug. Therefore, in the present study, hectorite has been used for the delivery of two hydrophobic molecules; chemotherapeutic drug DDP [Cis-diamminedichloroplatinum (II)] and natural photosensitizer hypocrellin A (HA) for combinatorial therapy. The experimental results has been fitted to l-type (Langmuir) adsorption isotherm. The adsorption isotherm showed higher affinity of DDP toward the hectorite with almost comparable CEC value of initial silicate layer of hectorite. The loading of drug into nanoclay was further characterize by using SEM, FTIR, XRD, DLS and TGA, which suggest the presence of DDP molecules in between the interparticle and photosensitizer on the surface of nanoclay. After, adsorption the characteristic properties such as photostability and photobleaching of HA has been significantly improved. The photobleaching efficiencies of free HA and Ht-DDP-HA was 72.8 and 55.2%, respectively, and the ¹O₂ generation quantum yield was 0.86. The in vitro release rate is twofold higher in the presence of laser light and ultrasonic waves. The drug release profile was found to be best fitted by Higuchi square root model, which implicate that release of drug from matrix as square root of time-dependent process and diffusion controlled. The in vitro efficacy of Ht-DDP-HA showed the potent anti-cancerous ability due to combinatorial effect by 2.01 and 1.69 fold times higher efficacy than free DDP and HA, respectively. Flow cytometry data shows the synergistic effect of combined therapy by showing maximum apoptotic and necrotic percentage.

锂蒙脱石是三八面体粘土矿物，具有独特的表面反应性和吸附性、阳离子交换能力以及在水中容易分层成单个纳米层的特性。因此，锂蒙脱石可以成为一种具有生物相容性、高有效负载和药物控释的有前途的纳米载体。因此，在本研究中，锂蒙脱石已被用于传递两种疏水性分子；化疗药物DDP[顺二氨二氯铂(II)]与天然光敏剂竹红菌素A(HA)联合治疗。实验结果符合l型（Langmuir）吸附等温线。吸附等温线显示DDP对锂蒙脱石具有更高的亲和力，并且与锂蒙脱石初始硅酸盐层的CEC值几乎相当。通过使用 SEM、FTIR、XRD、DLS 和 TGA 进一步表征了纳米粘土中药物的负载，这表明纳米粘土表面的颗粒间和光敏剂之间存在 DDP 分子。吸附后HA的光稳定性、光漂白性等特性得到显着改善。游离HA和Ht-DDP-HA的光漂白效率分别为72.8%和55.2%，¹ O ₂产生量子产率为0.86。在激光和超声波存在下，体外释放率高出两倍。发现药物释放曲线最适合 Higuchi 平方根模型，这意味着药物从基质中的释放是时间依赖性过程和扩散控制的平方根。Ht-DDP-HA 的体外功效显示出由于组合作用而具有强大的抗癌能力，其功效分别比游离 DDP 和 HA 高 2.01 倍和 1.69 倍。流式细胞术数据通过显示最大凋亡和坏死百分比来显示联合治疗的协同效应。