<br>内在双发射胰岛素封端的 Au/Ag 纳米团簇作为单一比率纳米探针，用于可逆检测 pH 值和温度以及细胞成像,Biosensors and Bioelectronics

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内在双发射胰岛素封端的 Au/Ag 纳米团簇作为单一比率纳米探针，用于可逆检测 pH 值和温度以及细胞成像
Biosensors and Bioelectronics ( IF 10.7 ) Pub Date : 2024-01-23 , DOI: 10.1016/j.bios.2024.116064
Mojtaba Shamsipur ₁ , Elaheh Babaee ₁ , Mohammad-Bagher Gholivand ₁ , Fatemeh Molaabasi ₂ , Behnam Hajipour-Verdom ₃ , Mosslim Sedghi ₄

Affiliation

pH 值和温度是细胞和环境的两个重要特征。这些不仅在细胞功能的良好调节中，而且在诊断和治疗中都发挥着关键作用。蛋白质保护的双金属纳米团簇广泛用于生物传感器的构建。然而，具有双重本征发射的胰岛素稳定的金银纳米团簇尚未得到研究。在这项工作中，首次以简单、绿色的一次性方式合成了双发射胰岛素模板金银纳米簇（Ins(Au/Ag)NCs）。所制备的 NC 的两个发射波长集中在 410 和 630 nm，在一种激发波长 (330 nm) 下激发。这两个发射峰分别归属于二酪氨酸交联形成和双金属纳米团簇。进一步分析表明，Ins(Au/Ag)NCs 的每个发射带响应一个变量，而另一个峰保持恒定；对于蓝色和红色发射波长，分别观察了 pH 依赖性和热响应性。所制备的具有固有双发射特征的纳米探针用于这些参数的比率测定，每个参数都具有来自另一个的离散响应。获得了pH 6.0–9.0和温度1至71°C的线性范围，该范围包含pH和温度的生理范围，并提供细胞内传感和成像能力。所制备的 NCs 探针表现出优异的生物相容性和细胞膜渗透性，因此成功应用于 HeLa 和 HFF 细胞中的直接比例 pH 和温度探针。更有趣的是，这种双发射纳米探针能够区分癌细胞和正常细胞。

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Intrinsic dual emissive insulin capped Au/Ag nanoclusters as single ratiometric nanoprobe for reversible detection of pH and temperature and cell imaging

pH and temperature are two important characteristics in cells and the environment. These, not only in the well-done regulation of cell functions but also in diagnosis and treatment, have a key role. Protein-protected bimetallic nanoclusters are abundantly used in the building of biosensors. However, insulin-stabilized Au–Ag nanoclusters with dual intrinsic emission have not been investigated yet. In this work, Dual emissive insulin templated Au–Ag nanocluster (Ins(Au/Ag)NCs) were first synthesized in a simple and green one-put manner. The two emission wavelengths of, as-prepared NCs centered at 410 and 630 nm, excited in one excitation wavelength (330 nm). These two emission peaks were assigned to the di-Tyrosine cross-linked formation and bimetallic nanoclusters respectively. Further analysis displayed that each emission band of Ins(Au/Ag)NCs responded to one variable whilst another peak remained constant; For blue and red emission wavelengths, pH dependency and thermo-responsibility were observed respectively. As-prepared nanoprobe with the intrinsic dual emissive feature was used for ratiometric determination of these parameters, each with a discrete response from another. The linear range of 6.0–9.0 for pH and 1 to 71 °C for temperature was obtained, which comprises the physiological range of pH and temperature and afforded intracellular sensing and imaging capability. As-prepared NCs probe show excellent biocompatibility and cell membrane permeability, and so were successfully applied as direct ratiometric pH and temperature probes in HeLa and HFF cells. More interestingly, this dual emissive nanoprobe is capable of distinguishing cancer cells from normal ones.

更新日期：2024-01-23

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