<br>全氟辛酸诱导的免疫毒性导致菲律宾蛤仔转录、代谢和肠道微生物群变化的综合证据,Science of the Total Environment

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全氟辛酸诱导的免疫毒性导致菲律宾蛤仔转录、代谢和肠道微生物群变化的综合证据
Science of the Total Environment ( IF 8.2 ) Pub Date : 2024-01-23 , DOI: 10.1016/j.scitotenv.2024.170341
Fengling Li ₁ , Xiuqiong Gong ₂ , Yang Zhou ₂ , Qianqian Geng ₁ , Yanhua Jiang ₁ , Lin Yao ₁ , Meng Qu ₁ , Zhijun Tan ₃

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全氟辛酸 (PFOA) 是一种具有生物累积性的有毒污染物，由于其在全球环境中普遍存在且持续存在，因此成为一个重大的公共卫生问题。很少有研究评估 PFOA 对免疫系统的不利影响，对于软体动物尤其如此。在这里，通过代谢组、微生物组和转录组的综合分析来评估 PFOA 对免疫系统的相关影响，为与免疫毒性相关的可能机制提供证据。 PFOA 暴露导致血淋巴细胞内与免疫调节功能相关的几种重要代谢物发生明显变化，同时也改变了关键代谢途径，包括脂质、不饱和脂肪酸 (UFA) 和胆汁酸 (BA) 的代谢途径。接触 PFOAs 后，肠道细菌群落也发生明显变化，与肠道炎症相关的主要微生物群发生变化。分子分析提供了一致的结果，其中免疫相关基因的表达显着改变。多“组学”分析的整合表明，TLR/MyD88/NF-kB 通路以及 PI3K-Akt-mTOR 通路、PPAR 介导的脂质代谢和自噬信号通路可能在启动免疫毒性作用中发挥重要作用。接触 PFOA 后。这些结果进一步证明PFOA暴露可导致免疫功能障碍，也为PFAS改变双壳类免疫功能的机制提供了新的见解。

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Integrated evidence of transcriptional, metabolic, and intestinal microbiota changes in Ruditapes philippinarum due to perfluorooctanoic acid-induced immunotoxicity

Perfluorooctanoic acid (PFOA) is a toxic pollutant that bioaccumulates and is a significant public health concern due to its ubiquitous and persistent occurrence in global environments. Few studies have evaluated the adverse effects of PFOA on immune system, and this is particularly true for mollusks. Here, the PFOA-associated effects on immune system were evaluated in using integrated analysis of metabolomes, microbiomes, and transcriptomes, providing evidence for possible mechanisms related to immunotoxicity. PFOA exposure caused clear variation in several important metabolites related to immune regulatory function within the haemolyph from , while also altering key metabolic pathways, including those of lipids, unsaturated fatty acids (UFAs), and bile acids (BAs). After exposure to PFOAs, intestinal bacterial communities also clearly changed, with the predominant microflora becoming and that are related to intestinal inflammation. Molecular analyses provided consistent results, wherein the expression of immune-related genes was significantly altered. Integration of the multi-‘omics’ analyses suggested that the TLR/MyD88/NF-kB pathway, along with PI3K-Akt-mTOR pathway, PPAR-mediated lipid metabolism and the autophagy signaling pathway, likely play important roles in initiating immunotoxic effects in after PFOA exposure. These results provide further evidence that PFOA exposure can lead to immunologic dysfunction and also provide new insights into the mechanisms of PFAS alteration of bivalve immune function.

更新日期：2024-01-23

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