<br>新月柱藻和 Tetraselmis suecica 生物膜中的微藻 EPS 抑制细菌粘附,Applied Microbiology and Biotechnology

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新月柱藻和 Tetraselmis suecica 生物膜中的微藻 EPS 抑制细菌粘附
Applied Microbiology and Biotechnology ( IF 3.9 ) Pub Date : 2024-01-23 , DOI: 10.1007/s00253-023-12960-z
Julia Mougin _{1,

2} , Anne-Sophie Pavaux _{1,

2} , Andrea Fanesi ₂ , Julien Lopez ₃ , Eric Pruvost ₃ , Freddy Guihéneuf ₄ , Antoine Sciandra ₃ , Romain Briandet ₁ , Filipa Lopes ₂

Affiliation

抽象的

在食品工业中，细菌病原体的成功定植和持久性始于其粘附到表面，然后是引起公共卫生问题的成熟生物膜的空间发展。用天然抑制剂降低细菌沉降是传统防污处理的一种有前景的替代方案，传统防污处理通常基于化学杀菌剂，而化学杀菌剂会导致抗菌素耐药性负担日益加重。在本研究中，筛选了从新月柱藻（C 组分）和Tetraselmis suecica （富含不溶性鳞片结构的 Ta 组分和富含可溶性 EPS 的 Tb 组分）微藻生物膜中提取的三种胞外聚合物 (EPS) 组分的抗粘附特性，针对属于大肠杆菌、金黄色葡萄球菌、肠沙门氏菌亚种的八种人类食源性病原体。肠杆菌和单核细胞增生李斯特氏菌。结果表明，Ta 级分是最有效的，可显着减少大肠杆菌、金黄色葡萄球菌和单核细胞增生李斯特菌三种菌株的数量。总体而言，不同组分的聚苯乙烯表面上的 EPS 涂层增加了载体的亲水特性。根据 HPLC 和 ATR-FTIR 分析，不同涂层表面上细菌粘附的差异可以通过结构和物理化学 EPS 成分的一些差异来解释。有趣的是，虽然 Ta 和 Tb 组分是从相同的微藻培养物中提取的，但观察到了不同的粘附模式，凸显了提取过程的重要性。总体而言，研究结果表明，从微藻光合生物膜中提取的 EPS 可以对食源性病原体表现出抗粘附作用，并有助于为食品工业开发可持续且无毒的抗粘附表面。

关键点

•来自基于生物膜的C. closterium/T 培养物的EPS。 suecica 进行了表征。

•从 T. suecica 生物膜中提取的微藻 EPS 显示出细菌抗粘附作用。

• 抗粘附作用是菌株特异性的，并且影响革兰氏- 和革兰氏+ 细菌。

"点击查看英文标题和摘要"

Bacterial adhesion inhibition by microalgal EPSs from Cylindrotheca closterium and Tetraselmis suecica biofilms

Abstract

In the food industry, successful bacterial pathogen colonization and persistence begin with their adhesion to a surface, followed by the spatial development of mature biofilm of public health concerns. Compromising bacterial settlement with natural inhibitors is a promising alternative to conventional anti-fouling treatments typically based on chemical biocides that contribute to the growing burden of antimicrobial resistance. In this study, three extracellular polymeric substance (EPS) fractions extracted from microalgae biofilms of Cylindrotheca closterium (fraction C) and Tetraselmis suecica (fraction Ta rich in insoluble scale structure and fraction Tb rich in soluble EPS) were screened for their anti-adhesive properties, against eight human food-borne pathogens belonging to Escherichia coli, Staphylococcus aureus, Salmonella enterica subsp. enterica, and Listeria monocytogenes species. The results showed that the fraction Ta was the most effective inducing statistically significant reduction for three strains of E. coli, S. aureus, and L. monocytogenes. Overall, EPSs coating on polystyrene surfaces of the different fractions increased the hydrophilic character of the support. Differences in bacterial adhesion on the different coated surfaces could be explained by several dissimilarities in the structural and physicochemical EPS compositions, according to HPLC and ATR-FTIR analysis. Interestingly, while fractions Ta and Tb were extracted from the same microalgal culture, distinct adhesion patterns were observed, highlighting the importance of the extraction process. Overall, the findings showed that EPS extracted from microalgal photosynthetic biofilms can exhibit anti-adhesive effects against food-borne pathogens and could help develop sustainable and non-toxic anti-adhesive surfaces for the food industry.