In this work, iron oxide (Fe₃O₄) magnetic nanoparticles (MNPs) and graphene oxide (GO) nanosheets were prepared via the co-precipitation technique and the Modified Hummer method. Fe₃O₄ MNPs and GO nanosheets were combined to prepare Fe₃O₄/GO nanocomposite and subsequently conjugated with Digitonin (DIG) in order to obtain a dual-targeted delivery system based on DIG/Fe₃O₄/GO nanocomposite. SEM images reveal the presence of Fe₃O₄ MNPs at a scale of 100 nm, exhibiting dispersion between the GO nanosheets. Aggregation of the DIG/Fe₃O₄/GO nanocomposite was observed at various size scales. The XRD structural analysis confirms the crystal structure of the prepared samples. The Fe₃O₄ MNPs demonstrated the main XRD-diffracted peaks. Also, GO nanosheets exhibit crystalline characteristics on the (001) and (002) planes. The predominant peaks observed in the DIG/GO/Fe₃O₄ nanocomposite are attributed to the crystal phases of Fe₃O₄ MNPs. The FT-IR vibrational modes observed in the GO/DIG/Fe₃O₄ nanocomposite indicate the presence of crosslinking between GO nanosheet layers and the Fe₃O₄ MNPs. The antioxidant activity of the prepared samples was measured and the DIG/GO/Fe₃O₄ nanocomposite demonstrated a significantly high antioxidant activity in both 2-diphenyl-1-picrylhydrazyl (DPPH^·) and 2,2-azino-bis-3-ethylbenzthiazoline-6-sulfonic acid (ABTS^·+) tests.

在这项工作中，通过共沉淀技术和改进的Hummer方法制备了氧化铁（Fe ₃ O _{4 ）磁性纳米颗粒（MNP）和氧化石墨烯（GO）纳米片。}将Fe ₃ O ₄ MNPs和GO纳米片结合制备Fe ₃ O ₄ /GO纳米复合材料，随后与洋地黄皂苷(DIG)缀合，以获得基于DIG/Fe ₃ O ₄ /GO纳米复合材料的双靶向递送系统。SEM 图像显示存在100 nm 尺度的Fe ₃ O _{4 MNP，并表现出 GO 纳米片之间的分散性。}观察到不同尺寸尺度的DIG/Fe ₃ O _{4 /GO纳米复合材料的聚集。}XRD结构分析证实了所制备样品的晶体结构。Fe ₃ O ₄ MNP 显示出主要的 XRD 衍射峰。此外，GO 纳米片在 (001) 和 (002) 面上表现出结晶特性。_{在 DIG/GO/Fe 3} O ₄纳米复合材料中观察到的主峰归因于 Fe ₃ O ₄ MNP的晶相。在GO/DIG/Fe ₃ O _{4纳米复合材料中观察到的FT-IR振动模式表明GO纳米片层和Fe 3} O ₄ MNP之间存在交联。对所制备样品的抗氧化活性进行了测量，发现 DIG/GO/Fe ₃ O ₄纳米复合材料在 2-二苯基-1-三硝基苯肼 (DPPH ^· ) 和 2,2-azino-bis-3-中均表现出显着高的抗氧化活性。乙基苯并噻唑啉-6-磺酸（ABTS ^·+）测试。