Salivary Th17 cytokine, human β-defensin 1–3, and salivary scavenger and agglutinin levels in Crohn’s disease,Clinical Oral Investigations

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Salivary Th17 cytokine, human β-defensin 1–3, and salivary scavenger and agglutinin levels in Crohn’s disease
Clinical Oral Investigations ( IF 3.1 ) Pub Date : 2024-01-22 , DOI: 10.1007/s00784-024-05509-5
Ulvi Kahraman Gürsoy ₁ , Mervi Gürsoy _{1,

2} , Vuokko Loimaranta ₁ , Jaana Rautava _{3,

4}

Affiliation

Objectives

Crohn’s disease patients, who are prone to develop periodontal diseases, may carry genetic defects in their Th17 cytokine, human beta-defensin (hBD) 1–3, and salivary and scavenger agglutinin (SALSA) expressions. Biochemical composition of saliva reflects the oral consequences of systemic immune response modifications. Our aim was to evaluate the salivary Th17 cytokine, epithelial hBD 1–3, and SALSA levels in relation to Crohn’s disease.

Materials and methods

This cross-sectional study included 42 Crohn’s disease patients and 34 systemically healthy controls. Periodontal and dental indexes were measured, and stimulated saliva samples were collected. Salivary Th17 cytokine levels were analyzed by multiplex technique, and hBD 1–3 and SALSA levels by enzyme-linked immunosorbent assay.

Results

There were 19 gingivitis and 11 initial periodontitis patients in the Crohn’s disease group, and 15 gingivitis and 4 initial periodontitis in the control group. In comparison to controls, higher salivary Th17 cytokine levels were observed in Crohn’s disease patients. No statistical difference was observed between Crohn’s disease and control groups in terms of their salivary hBD 1–3 and SALSA levels. Based on the regression analysis, there is no independent association between Crohn’s disease and salivary Th17 cytokine levels.

Conclusions

Crohn’s disease does not relate to salivary antimicrobial hBD 1–3 or SALSA levels. While Crohn’s disease patients have higher salivary Th17 cytokine levels in comparison to systemically healthy controls, an independent association between Crohn’s disease and Th17 cytokine profile is still missing.

Clinical relevance

Diminished Th17 cytokine response in Crohn’s disease, which might be related to genetic susceptibility, can be also visualized in saliva.

中文翻译：

克罗恩病中的唾液 Th17 细胞因子、人 β-防御素 1-3 以及唾液清道夫和凝集素水平

目标

克罗恩病患者容易患牙周病，他们的 Th17 细胞因子、人 β-防御素（hBD） 1-3 以及唾液和清道夫凝集素（SALSA）表达可能携带遗传缺陷。唾液的生化成分反映了全身免疫反应修饰的口腔后果。我们的目的是评估与克罗恩病相关的唾液 Th17 细胞因子、上皮 hBD 1-3 和 SALSA 水平。

材料和方法

这项横断面研究包括 42 名克罗恩病患者和 34 名全身健康的对照者。测量牙周和牙齿指标，并收集刺激的唾液样本。通过多重技术分析唾液 Th17 细胞因子水平，通过酶联免疫吸附法分析 hBD 1-3 和 SALSA 水平。

结果

克罗恩病组有 19 例牙龈炎和 11 例初始牙周炎患者，对照组有 15 例牙龈炎和 4 例初始牙周炎患者。与对照组相比，在克罗恩病患者中观察到较高的唾液 Th17 细胞因子水平。克罗恩病组和对照组在唾液 hBD 1-3 和 SALSA 水平方面未观察到统计学差异。根据回归分析，克罗恩病与唾液 Th17 细胞因子水平之间没有独立关联。