Aqueous distillate of mature leaves of Vernonia zeylanica (L.) Less. and Mallotus repandus (Rottler) Müll. Arg. cued from traditional medicine exhibits rapid wound healing properties,Journal of Ethnopharmacology

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Aqueous distillate of mature leaves of Vernonia zeylanica (L.) Less. and Mallotus repandus (Rottler) Müll. Arg. cued from traditional medicine exhibits rapid wound healing properties
Journal of Ethnopharmacology ( IF 4.8 ) Pub Date : 2024-01-20 , DOI: 10.1016/j.jep.2024.117763
Praneeth Ratnayake ₁ , Udaya Samaratunga ₂ , Inoka Perera ₁ , Jayamini Seneviratne ₃ , Preethi Udagama ₁

Affiliation

Ethnopharmacological relevance

Sri Lankan traditional medicine uses Vernonia zeylanica and Mallotus repandus broadly for the treatment of a multitude of disease conditions, including wound healing.

Aim of the study

We aimed to scientifically validate the safety and efficacy of wound healing of an aqueous distillate of Vernonia zeylanica and Mallotus repandus (ADVM) mature leaves, tested on primary human dermal fibroblasts.

Materials and methods

Human dermal fibroblasts isolated from clinical waste from circumcision surgery were characterized by flowcytometry and trilineage differentiation. The MTT dye reduction assay, and the ex vivo wound healing scratch assay established wound healing properties of ADVM using the primary human dermal fibroblast cell line. Upregulation of genes associated with wound healing (MMP3, COL3A1, TGFB1, FGF2) were confirmed by RT qPCR. GC-MS chromatography evaluated the phytochemical composition of ADVM.

Results

Compared to the synthetic stimulant, β fibroblast growth factor, ADVM at 0.25% concentration on the primary dermal fibroblast cell line exhibited significant ex vivo, (i) 1.7-fold % cell viability (178.7% vs 304.3 %, p < 0.001), (ii) twofold greater % wound closure (%WC) potential (47.74% vs 80.11%, p < 0.001), and (iii) higher rate of % WC (3.251 vs 3.456 % WC/h, p < 0.05), sans cyto-genotoxicity. Up regulated expression of FGF2, TGFB1, COL3A1 and MMP3, genes associated with wound healing, confirmed effective stimulation of pathways of the three overlapping phases of wound healing (P < 0.05). GC-MS profile of ADVM characterized four methyl esters, which may be posited as wound healing phytochemicals.

Conclusions

Exceeding traditional medicine claims, the ex vivo demonstration of rapid skin regeneration, reiterated by upregulated expression of genes related to wound healing pathways, sans cytotoxicity, propounds ADVM, cued from traditional medicine, as a potential safe and effective natural stimulant for rapid wound-healing. Additionally, it may serve as an effective proliferative stimulant of dermal fibroblasts for cell therapy, with potential in reparative and regenerative therapy of skin disorders.

中文翻译：

斑斑鸠菊 (Vernonia zeylanica (L.)) 成熟叶的水馏分较少。和 Mallotus repandus (Rottler) Müll。精氨酸。源自传统医学的提示显示出伤口快速愈合的特性

民族药理学相关性

斯里兰卡的传统医学广泛使用斑鸠菊和斑鸠菊来治疗多种疾病，包括伤口愈合。

研究目的

我们的目的是科学地验证斑鸠菊和斑鸠菊(ADVM) 成熟叶的水蒸馏物伤口愈合的安全性和有效性，并在原代人真皮成纤维细胞上进行测试。

材料和方法

从包皮环切手术的临床废物中分离出的人真皮成纤维细胞通过流式细胞术和三系分化进行表征。 MTT 染料还原测定和离体伤口愈合划痕测定使用原代人真皮成纤维细胞系确定了 ADVM 的伤口愈合特性。 RT qPCR 证实了与伤口愈合相关的基因（ MMP3 、COL3A1、 TGFB1 、 FGF2 ）的上调。 GC-MS 色谱法评估了 ADVM 的植物化学成分。

结果

与合成刺激剂 β 成纤维细胞生长因子相比，0.25% 浓度的 ADVM 对原代真皮成纤维细胞系表现出显着的离体效果，(i) 细胞活力提高 1.7 倍（178.7% vs 304.3%，p < 0.001）， (ii) 伤口闭合百分比 (%WC) 潜力增加两倍（47.74% 与 80.11%，p < 0.001），以及 (iii) % WC 比率更高（3.251 与 3.456 % WC/h，p < 0.05），无细胞遗传毒性。与伤口愈合相关的基因FGF2 、 TGFB1 、 COL3A1和MMP3的表达上调，证实了对伤口愈合三个重叠阶段的途径的有效刺激（P < 0.05）。 ADVM 的 GC-MS 谱表征了四种甲酯，它们可能被认为是伤口愈合植物化学物质。