The design and fabrication of multifunctional hybrid hydrogels for various biomedical applications have sparked tremendous scientific interest and still remain a formidable challenge. Here, we report the construction of a multifunctional supramolecular hybrid hydrogel through a facile preparation route by co-assembling the L-phenylalanine-derivative (LPF) gelator with agarose (AG) and gallic acid (GA). The experimental analysis demonstrates that the components of hybrid hydrogel co-assembled to form a cross-linked interpenetrating network that is mainly formed through non-covalent interactions, thereby resulting in remarkable enhancement in rheological properties with an elastic modulus (G′) of 12715 Pa. Due to the inherent antimicrobial property of GA, this hybrid hydrogel exhibited antibacterial activities against both Gram-negative (Pseudomonas aeruginosa, Escherichia coli) and Gram-positive (Staphylococcus aureus) bacterial cells. Furthermore, the presence of GA enabled hybrid hydrogels to offer efficient antioxidant properties by scavenging free radicals such as DPPH· and ABTS·+. The cell adhesion, proliferation, and spreading behavior of L929 cells were significantly improved on the hybrid hydrogel substrate. These experimental findings suggest that this high-performance hybrid hydrogel possessing antibacterial, antioxidant, and biocompatible properties, opens new avenues for extensive applications in the biomedical field.

用于各种生物医学应用的多功能混合水凝胶的设计和制造引起了巨大的科学兴趣，但仍然是一个艰巨的挑战。在这里，我们报告了通过将 L-苯丙氨酸衍生物（LPF）凝胶剂与琼脂糖（AG）和没食子酸（GA）共组装的简便制备途径构建了多功能超分子杂化水凝胶。实验分析表明，杂化水凝胶的组分共同组装形成主要通过非共价相互作用形成的交联互穿网络，从而导致流变性能显着增强，弹性模量（G'）为12715 Pa由于GA固有的抗菌特性，这种混合水凝胶对革兰氏阴性（铜绿假单胞菌、大肠杆菌）和革兰氏阳性（金黄色葡萄球菌）细菌细胞均表现出抗菌活性。此外，GA 的存在使混合水凝胶能够通过清除 DPPH· 和 ABTS·+ 等自由基来提供有效的抗氧化特性。L929 细胞在混合水凝胶基底上的细胞粘附、增殖和铺展行为显着改善。这些实验结果表明，这种高性能混合水凝胶具有抗菌、抗氧化和生物相容性，为生物医学领域的广泛应用开辟了新途径。