Whey Protein Peptide Pro-Glu-Trp Ameliorates Hyperuricemia by Enhancing Intestinal Uric Acid Excretion, Modulating the Gut Microbiota, and Protecting the Intestinal Barrier in Rats,Journal of Agricultural and Food Chemistry

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Whey Protein Peptide Pro-Glu-Trp Ameliorates Hyperuricemia by Enhancing Intestinal Uric Acid Excretion, Modulating the Gut Microbiota, and Protecting the Intestinal Barrier in Rats
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2024-01-19 , DOI: 10.1021/acs.jafc.3c00984
Xiaofen Qi _{1,

2} , Yanfeng Ma ₃ , Kaifang Guan ₁ , Le Zhao ₁ , Ying Ma ₁ , Rongchun Wang ₁

Affiliation

Hyperuricemia (HUA) is a metabolic disorder characterized by an increase in the concentrations of uric acid (UA) in the bloodstream, intricately linked to the onset and progression of numerous chronic diseases. The tripeptide Pro-Glu-Trp (PEW) was identified as a xanthine oxidase (XOD) inhibitory peptide derived from whey protein, which was previously shown to mitigate HUA by suppressing UA synthesis and enhancing renal UA excretion. However, the effects of PEW on the intestinal UA excretion pathway remain unclear. This study investigated the impact of PEW on alleviating HUA in rats from the perspective of intestinal UA transport, gut microbiota, and intestinal barrier. The results indicated that PEW inhibited the XOD activity in the serum, jejunum, and ileum, ameliorated intestinal morphology changes and oxidative stress, and upregulated the expression of ABCG2 and GLUT9 in the small intestine. PEW reversed gut microbiota dysbiosis by decreasing the abundance of harmful bacteria (e.g., Bacteroides, Alloprevotella, and Desulfovibrio) and increasing the abundance of beneficial microbes (e.g., Muribaculaceae, Lactobacillus, and Ruminococcus) and elevated the concentration of short-chain fatty acids. PEW upregulated the expression of occludin and ZO-1 and decreased serum IL-1β, IL-6, and TNF-α levels. Our findings suggested that PEW supplementation ameliorated HUA by enhancing intestinal UA excretion, modulating the gut microbiota, and restoring the intestinal barrier function.

中文翻译：

乳清蛋白肽 Pro-Glu-Trp 通过增强肠道尿酸排泄、调节肠道菌群、保护大鼠肠道屏障改善高尿酸血症

高尿酸血症（HUA）是一种代谢性疾病，其特征是血液中尿酸（UA）浓度升高，与多种慢性疾病的发病和进展密切相关。三肽 Pro-Glu-Trp (PEW) 被鉴定为衍生自乳清蛋白的黄嘌呤氧化酶 (XOD) 抑制肽，此前研究表明其可通过抑制 UA 合成和增强肾脏 UA 排泄来减轻 HUA。然而，光能波对肠道UA排泄途径的影响仍不清楚。本研究从肠道UA转运、肠道微生物群和肠道屏障的角度探讨了光能波对缓解大鼠HUA的影响。结果表明，光能波抑制血清、空肠和回肠中的XOD活性，改善肠道形态变化和氧化应激，并上调小肠中ABCG2和GLUT9的表达。光能波通过减少有害细菌（如拟杆菌、同源菌属和脱磺弧菌）的丰度、增加有益微生物（如杆菌科、乳杆菌和瘤胃球菌）的丰度以及提高短链脂肪酸的浓度来逆转肠道微生物群失调。光能波上调了occludin和ZO-1的表达，并降低了血清IL-1β、IL-6和TNF-α水平。我们的研究结果表明，补充光能波可通过增强肠道 UA 排泄、调节肠道微生物群和恢复肠道屏障功能来改善 HUA。

更新日期：2024-01-19

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