<br>核桃蛋白降解获得的 Akt 激活肽对预防小鼠记忆障碍的作用,Food & Function

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核桃蛋白降解获得的 Akt 激活肽对预防小鼠记忆障碍的作用
Food & Function ( IF 5.1 ) Pub Date : 2024-01-20 , DOI: 10.1039/d3fo04479c
Like Lin ₁ , Cong Li ₁ , Yujiao Zhang ₁ , Li Zhang ₁ , Lu Gao ₁ , Lihua Jin ₁ , Yu Shu ₂ , Yehua Shen ₁

Affiliation

Akt 作为影响神经退行性疾病（包括 AD 和 PD）多种病理的中心蛋白，使用 Akt 激活剂是一种有前景的治疗策略。目前的研究表征了从核桃蛋白降解中获得的 Akt 激活肽（Glu-Pro-Glu-Val-Leu-Pro，EPEVLR）对D -gal 诱导的小鼠记忆障碍的影响。通过核桃蛋白水解、肽序列鉴定、分子对接筛选依次获得EPEVLR。小鼠的 MWM 测试表明，口服 EPEVLR（每天每公斤 80、200 和 400 毫克）可显着 ( p < 0.05) 逆转D -gal 诱导的记忆障碍。小鼠海马的 WB 测试证实 EPEVLR 可以通过促进 Akt 磷酸化来激活 Akt。此外，与 Akt 磷酸化相关的进一步表征（包括 TEM、ELISA 和免疫组织化学）显示，与模型组相比，Aβ 和 p-tau 水平较低，自噬体也较多。此外，EPEVLR 治疗显着增加了肠道微生物组中乳酸菌的丰度并减少了螺杆菌的丰度，并导致血清代谢物中 SCFA 的上调和 LPS 的下调。因此，摄入 EPEVLR 可以逆转认知障碍症状，可能与 Akt 的激活和肠道菌群途径的调节有关。食用含有 EPEVLR 的饮食有利于治疗认知功能障碍。

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Effects of an Akt-activating peptide obtained from walnut protein degradation on the prevention of memory impairment in mice

Akt acts as a central protein influencing multiple pathologies in neurodegenerative diseases including AD and PD, and using Akt activators is a promising management strategy. The current study characterized the effects of an Akt-activating peptide (Glu-Pro-Glu-Val-Leu-Pro, EPEVLR) obtained from walnut protein degradation on D-gal-induced memory impairment in mice. EPEVLR was obtained by hydrolysis of walnut proteins, identification of peptide sequences, and screening for molecular docking sequentially. The MWM test in mice indicated that the oral administration of EPEVLR (80, 200 and 400 mg per kg per day) significantly (p < 0.05) reversed D-gal-induced memory impairment. WB tests of the mouse hippocampus confirmed that EPEVLR could activate Akt by promoting its phosphorylation. In addition, further characterization (including TEM, ELISA, and immunohistochemistry) related to Akt phosphorylation showed lower Aβ and p-tau levels, as well as more autophagosomes than those in the model group. Moreover, the EPEVLR treatment significantly increased Lactobacillus abundance and reduced Helicobacter abundance in the gut microbiome and caused up-regulation of SCFAs and down-regulation of LPS of serum metabolites. Therefore, EPEVLR ingestion reversed cognitive impairment symptoms, possibly related to the activation of Akt and regulation of the intestinal flora pathway. Consumption of an EPEVLR-containing diet is beneficial for treating cognitive dysfunction.

更新日期：2024-01-20

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