PSME3 plays a significant role in tumor progression. However, the prognostic value of PSME3 in pan-cancer and its involvement in tumor immunity remain unclear. We conducted a comprehensive study utilizing extensive RNA sequencing data from the TCGA (The Cancer Genome Atlas) and GTEx (Genotype-Tissue Expression) databases. Our research revealed abnormal expression levels of PSME3 in various cancer types and unveiled a correlation between high PSME3 expression and adverse clinical outcomes, especially in cancers like liver cancer (LIHC) and lung adenocarcinoma (LUAD). Functional enrichment analysis highlighted multiple biological functions of PSME3, including its involvement in protein degradation, immune responses, and stem cell regulation. Moreover, PSME3 showed associations with immune infiltration and immune cells in the tumor microenvironment, indicating its potential role in shaping the cancer immune landscape. The study also unveiled connections between PSME3 and immune checkpoint expression, with experimental validation demonstrating that PSME3 positively regulates CD276. This suggests that PSME3 could be a potential therapeutic target in immunotherapy. Additionally, we predicted sensitive drugs targeting PSME3. Finally, we confirmed in both single-factor Cox and multiple-factor Cox regression analyses that PSME3 is an independent prognostic factor. We also conducted preliminary validations of the impact of PSME3 on cell proliferation and wound healing in liver cancer. In summary, our study reveals the multifaceted role of PSME3 in cancer biology, immune regulation, and clinical outcomes, providing crucial insights for personalized cancer treatment strategies and the development of immunotherapy.

中文翻译：

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PSME3综合分析：从泛癌分析到实验验证

PSME3 在肿瘤进展中发挥着重要作用。然而，PSME3在泛癌中的预后价值及其与肿瘤免疫的关系仍不清楚。我们利用来自 TCGA（癌症基因组图谱）和 GTEx（基因型组织表达）数据库的大量 RNA 测序数据进行了一项全面的研究。我们的研究揭示了各种癌症类型中 PSME3 的异常表达水平，并揭示了 PSME3 高表达与不良临床结果之间的相关性，特别是在肝癌 (LIHC) 和肺腺癌 (LUAD) 等癌症中。功能富集分析强调了 PSME3 的多种生物学功能，包括其参与蛋白质降解、免疫反应和干细胞调节。此外，PSME3 显示出与肿瘤微环境中的免疫浸润和免疫细胞的相关性，表明其在塑造癌症免疫景观中的潜在作用。该研究还揭示了 PSME3 与免疫检查点表达之间的联系，实验验证表明 PSME3 正向调节 CD276。这表明 PSME3 可能成为免疫疗法的潜在治疗靶点。此外，我们预测针对 PSME3 的敏感药物。最后，我们在单因素 Cox 和多因素 Cox 回归分析中证实 PSME3 是一个独立的预后因素。我们还初步验证了 PSME3 对肝癌细胞增殖和伤口愈合的影响。总之，我们的研究揭示了 PSME3 在癌症生物学、免疫调节和临床结果中的多方面作用，为个性化癌症治疗策略和免疫疗法的发展提供了重要的见解。