Background

In this experiment, we synthesized a new compound, 1-(4-chlorobenzoyl)-4-(dimethylamino) pyridin-1-ium chloride (SM-9), and examined its toxicity and anticancer activity in human colon cancer (HCT-116) cells.

Methods

We uncovered the underlying mechanisms of cell toxicity and apoptosis due to SM-9 compound exposure in HCT-116 cells by using the MTT assay and protein profiling array and measuring gene expression levels using reverse transcription-polymerase chain reaction.

Results

Our data show that the SM-9 compound activated the caspase-3, caspase-8, p21, p27, and p53 proteins involved in the apoptosis of HCT-116 cells, thereby inducing cytotoxicity, the formation of reactive oxygen species, and apoptosis. The results of this study show that the SM-9 compound has advantageous qualities and must be taken as an anticancer medication. We conclude that the SM-9 compound exerted an anticancer effect by inducing the apoptotic pathways in human colon cancer (HCT-116) cells.

中文翻译：

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1-(4-氯苯甲酰基)-4-(二甲氨基)吡啶-1-氯化鎓的合成及其对人结肠癌细胞的细胞毒性和凋亡测定

背景

在本实验中，我们合成了一种新化合物1-(4-氯苯甲酰基)-4-(二甲氨基)吡啶-1-氯化鎓(SM-9)，并检测了其对人结肠癌的毒性和抗癌活性(HCT-116) ） 细胞。

方法

我们通过使用 MTT 测定和蛋白质分析阵列并使用逆转录聚合酶链式反应测量基因表达水平，揭示了 HCT-116 细胞中 SM-9 化合物暴露引起的细胞毒性和细胞凋亡的潜在机制。

结果

我们的数据表明，SM-9 化合物激活参与 HCT-116 细胞凋亡的 caspase-3、caspase-8、p21、p27 和 p53 蛋白，从而诱导细胞毒性、活性氧的形成和细胞凋亡。这项研究的结果表明，SM-9化合物具有优越的品质，必须作为抗癌药物。我们得出结论，SM-9 化合物通过诱导人结肠癌细胞 (HCT-116) 细胞凋亡途径发挥抗癌作用。