Treating nitrogenous compounds in wastewater is a contemporary challenge, prompting novel approaches for ammonium (NH₄⁺) conversion to molecular nitrogen (N₂). This study explores the classic anaerobic ammonium oxidation process (Anammox) coupled to the iron-dependent anaerobic ammonium oxidation process (Feammox) in a sequential discontinuous bioreactor (SBR) for NH₄⁺ removal. Feammox and Anammox cultures were individually enriched and combined, optimizing the coupling, and identifying key variables influencing the enrichment process. Adding sodium acetate as a carbon source significantly reduces Fe³⁺ to Fe²⁺, indicating Feammox activity. Both Anammox and Feammox processes were successfully operated in SBRs, achieving efficient NH₄⁺ removal (Anammox: 64.6 %; Feammox: 43.4 %). Combining these pathways in a single SBR enhances the NH₄⁺ removal capacity of 50.8 %, improving Feammox efficiency. The Feammox process coupled with Anammox may generate the nitrite (NO₂^–) needed for Anammox. This research contributes to biotechnological advancements for sustainable nitrogenous compound treatment in SBRs.

处理废水中的含氮化合物是一项当代挑战，催生了将铵 (NH ₄ ⁺ ) 转化为分子氮 (N ₂ ) 的新方法。本研究探讨了在顺序间断生物反应器 (SBR) 中将经典厌氧氨氧化过程 (Anammox) 与铁依赖性厌氧氨氧化过程 (Feammox) 耦合以去除 NH ₄ ⁺ 。 Feammox 和 Anammox 培养物分别进行富集和组合，优化耦合，并确定影响富集过程的关键变量。添加乙酸钠作为碳源显着地将Fe ³⁺还原为Fe ²⁺ ，表明Feamox活性。 Anammox 和 Feammox 工艺均在 SBR 中成功运行，实现了高效的 NH ₄ ⁺去除（Anammox：64.6 %；Feammox：43.4 %）。将这些途径组合在单个 SBR 中可将 NH ₄ ⁺去除能力提高 50.8%，从而提高 Feammox 效率。 Feammox 工艺与 Anammox 结合可产生 Anammox 所需的亚硝酸盐 (NO ₂ ^– )。这项研究有助于 SBR 中可持续含氮化合物处理的生物技术进步。