Parasitology Research ( IF 1.8 ) Pub Date : 2024-01-15 , DOI: 10.1007/s00436-024-08122-3 Luis A Gomez-Puerta 1 , Javier Jara-Vila 2 , Miguel Anampa 3 , Julia M Garayar 1 , Wendy Rojas-Anticona 2 , Hugo Castillo 1
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Abstract
Cases of sarcoptic mange in the wildlife population have been increasing around the world in recent years. In this study, we report the first case of severe sarcoptic mange in two Andean foxes (Lycalopex culpaeus) and molecularly analyze the collected mites. The National Forestry and Wildlife Service of Lima, Peru, found an adult male Andean fox in the province of Callao in August 2022. The veterinarians decided to euthanize the fox due to the severity of the mange. In August 2023, an adult male Andean fox was found dead in the province of Huaral by veterinarians. Both foxes were sent to the veterinary school in Lima for necropsy. Skin samples from different body zones were digested in buffer lysis, and mites were detected in the tissue samples. A morphological diagnosis identified the mites as Sarcoptes scabiei. The mites from both foxes had the same nuclear (ITS2) and mitochondrial (cox1) genetic marker sequences as the S. scabiei sequences from dogs, vicunas, Andean foxes, and water buffalos recorded in GenBank. Unlike ITS2, phylogenetic analysis of S. scabiei cox1 showed host-related nucleotide sequence polymorphisms. Future molecular studies of S. scabiei from different hosts and localities will be necessary to better understand the transmission of this disease in Andean foxes.
中文翻译：
引起秘鲁两只安第斯狐狸 (Lycalopex culpaeus) 严重疥癣的疥螨的分子特征
抽象的
近年来，世界各地野生动物中的疥癣病例不断增加。在这项研究中，我们报告了两只安第斯狐狸（ Lycalopex culpaeus ）的第一例严重疥螨病，并对收集到的螨虫进行了分子分析。 2022年8月，秘鲁利马国家林业和野生动物管理局在卡亚俄省发现了一只成年雄性安第斯狐狸。由于疥癣严重，兽医决定对这只狐狸实施安乐死。 2023年8月，兽医在瓦拉尔省发现一只成年雄性安第斯狐狸死亡。两只狐狸都被送往利马兽医学校进行尸检。来自不同身体部位的皮肤样本在缓冲液裂解中被消化，并在组织样本中检测到螨虫。形态学诊断确认该螨虫为Sarcoptes scabiei 。两只狐狸的螨虫与 GenBank 中记录的狗、骆马、安第斯狐狸和水牛的疥螨序列具有相同的核 ( ITS2 ) 和线粒体 ( cox1 ) 遗传标记序列。与ITS2不同， S. scabiei cox1的系统发育分析显示宿主相关的核苷酸序列多态性。为了更好地了解这种疾病在安第斯狐狸中的传播，未来有必要对来自不同宿主和地点的疥癣菌进行分子研究。
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