This work investigated the effects of pine-cone biochar (PBC) as an additive on anaerobic digestion process of liquor wastewater. Results showed that the best results were achieved at a dose of 2.5 g/L, with a peak cumulative methane production of 71%. PBC facilitated the hydrolysis process and favored the microbial secretion of humic acids, increasing the activity of the electron transport system and the conductivity of the sludge. Microbial analysis demonstrated that PBC enriched the growth of Geobacter, Methanosarcina and Methanosaeta groups participated in direct interspecies electron transfer, and Latescibacteria, chlamydiae and calditrichaeota groups as key microorganisms in the hydrolysis process. Finally, XRD and FT-IR analyses showed that the compositions and functional groups of PBC could accelerate electron transport while acting as a buffer. The techniques and findings showcased here can serve as a helpful benchmark toward enhancing the effectiveness of anaerobic digestion during the processing of liquor wastewater.

中文翻译：

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松果生物炭强化废液厌氧消化产甲烷新策略


本工作研究了松果生物炭（PBC）作为添加剂对含酒废水厌氧消化过程的影响。结果表明，剂量为2.5 g/L时效果最佳，累积甲烷产量峰值为71%。 PBC促进了水解过程，有利于微生物分泌腐殖酸，增加了电子传递系统的活性和污泥的电导率。微生物分析表明，PBC丰富了参与直接种间电子转移的地杆菌属、甲烷八叠球菌属和甲烷菌群的生长，而乳杆菌属、衣原体和热毛菌群是水解过程中的关键微生物。最后，XRD 和 FT-IR 分析表明，PBC 的成分和官能团可以加速电子传输，同时充当缓冲剂。这里展示的技术和研究结果可以作为提高含酒废水处理过程中厌氧消化效率的有用基准。