The polymorphism T1470A of the SLC16A1 gene is associated with the lactate and ventilatory thresholds but not with fat oxidation capacity in young men,European Journal of Applied Physiology

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The polymorphism T1470A of the SLC16A1 gene is associated with the lactate and ventilatory thresholds but not with fat oxidation capacity in young men
European Journal of Applied Physiology ( IF 2.8 ) Pub Date : 2024-01-12 , DOI: 10.1007/s00421-023-05407-w
Isaac A Chávez-Guevara ₁ , Everardo González-Rodríguez ₂ , Verónica Moreno-Brito ₂ , Jorge A Pérez-León ₃ , Francisco J Amaro-Gahete _{4,

5,

6} , Marina Trejo-Trejo ₇ , Arnulfo Ramos-Jiménez ₈

Affiliation

Purpose

To examine the association of the single nucleotide polymorphism A1470T in the SLC16A1 gene with blood lactate accumulation during a graded exercise test and its associated metaboreflex.

Methods

Forty-six Latin-American men (Age: 27 ± 6 years; Body fat: 17.5 ± 4.7%) performed a graded exercise test on a treadmill for the assessment of maximal oxygen uptake (VO_2max), lactate threshold (LT), ventilatory threshold (VT) and the exercise intensity corresponding to maximal fat oxidation rate (FATmax), via capillary blood samples and indirect calorimetry. Genomic DNA was extracted from a peripheral blood sample. Genotyping assay was carried out by real-time polymerase chain reaction to identify the A1470T polymorphism (rs1049434).

Results

Genotypes distribution were in Hardy–Weinberg equilibrium (X² = 5.6, p > 0.05), observing allele frequencies of 0.47 and 0.53 for the A and T alleles, respectively. No difference in VO_2max, body composition nor FATmax were observed across genotypes, whereas carriers of the TT genotype showed a higher LT (24.5 ± 2.2 vs. 15.6 ± 1.7 mL kg⁻¹ min⁻¹, p < 0.01) and VT in comparison to carriers of the AA + AT genotypes (32.5 ± 3.3 vs. 21.7 ± 1.5 mL kg⁻¹ min⁻¹, p < 0.01). Both, VO_2max and the A1470T polymorphism were positively associated to the LT (R² = 0.50, p < 0.01) and VT (R² = 0.55, p < 0.01). Only VO_2max was associated to FATmax (R² = 0.39, p < 0.01).

Conclusion

Independently of cardiorespiratory fitness, the A1470T polymorphism is associated to blood lactate accumulation and its associated ventilatory response during submaximal intensity exercise. However, the A1470 polymorphism does not influence fat oxidation capacity during exercise in young men.

中文翻译：

SLC16A1 基因的多态性 T1470A 与年轻男性的乳酸阈值和通气阈值相关，但与脂肪氧化能力无关

目的

旨在检查 SLC16A1 基因中的单核苷酸多态性 A1470T 与分级运动测试期间血乳酸积累及其相关代谢反射的关联。

方法

46 名拉丁美洲男性（年龄：27 ± 6 岁；体脂：17.5 ± 4.7%）在跑步机上进行分级运动测试，以评估最大摄氧量 (VO _2max )、乳酸阈值 (LT)、通气量通过毛细血管血样和间接量热法确定阈值 (VT) 和与最大脂肪氧化率 (FATmax) 相对应的运动强度。从外周血样本中提取基因组 DNA。通过实时聚合酶链式反应进行基因分型测定，以鉴定 A1470T 多态性（rs1049434）。

结果

基因型分布处于 Hardy-Weinberg 平衡（ X ² = 5.6， p > 0.05），观察到 A 和 T 等位基因的等位基因频率分别为 0.47 和 0.53。不同基因型的 VO _2max 、身体成分和 FATmax 没有差异，而 TT 基因型携带者相比之下表现出更高的 LT（24.5 ± 2.2 对比 15.6 ± 1.7 mL kg ⁻¹ min ⁻¹ ， p < 0.01）和 VT与 AA + AT 基因型携带者相比（32.5 ± 3.3 与 21.7 ± 1.5 mL kg ^-1 min ^-1 ， p < 0.01）。 VO _2max和 A1470T 多态性均与 LT ( R ² = 0.50, p < 0.01) 和 VT ( R ² = 0.55, p < 0.01) 呈正相关。仅 VO _2max与 FATmax 相关（ R ² = 0.39， p < 0.01）。