Discovery, Identification, and Mode of Action of Phenolics from Marine-Derived Fungus Aspergillus ustus as Antibacterial Wilt Agents,Journal of Agricultural and Food Chemistry

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Discovery, Identification, and Mode of Action of Phenolics from Marine-Derived Fungus Aspergillus ustus as Antibacterial Wilt Agents
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2024-01-12 , DOI: 10.1021/acs.jafc.3c07826
Jingjing Xue ₁ , Xiaopeng Guo _{1,

2} , Guangxin Xu ₁ , Xi Chen ₁ , Lihang Jiao ₁ , Xixiang Tang ₁

Affiliation

The bacterial wilt caused by Ralstonia solanacearum seriously affects crop yield and safety and is difficult to control. Biological activity-guided screening led to the isolation of 11 phenolic compounds including three undescribed compounds (carnemycin H–I and stromemycin B) from the secondary metabolites of a marine-derived Aspergillus ustus. One new compound is an unusual phenolic dimer. Their structures were elucidated by comprehensive spectroscopic data and J-based configurational analysis. The antibacterial activities of the isolated compounds against R. solanacearum were evaluated. Compound 3 exhibited excellent inhibitory activity with an MIC value of 3 μg/mL, which was comparable to that of streptomycin sulfate. Additionally, 3 significantly changed the morphology and inhibited the activity of succinate dehydrogenase (SDH) to interfere with the growth of R. solanacearum. Molecular docking was conducted to clarify the potential mechanisms of compound 3 with SDH. Further in vivo experiments demonstrated that 3 could remarkably inhibit the occurrence of bacterial wilt on tomatoes.

中文翻译：

海洋源性真菌曲霉中酚类化合物的发现、鉴定及其作用模式作为抗菌青枯病剂

青枯雷尔斯顿菌引起的青枯病严重影响作物产量和安全，且防治难度大。以生物活性为指导的筛选从海洋来源的曲霉菌的次级代谢产物中分离出了 11 种酚类化合物，其中包括三种未描述的化合物（肉霉素 H-I 和基质霉素 B）。一种新化合物是一种不寻常的酚类二聚体。通过综合光谱数据和基于J 的构型分析阐明了它们的结构。评估了分离的化合物对青枯菌的抗菌活性。化合物3表现出优异的抑制活性，MIC值为3 μg/mL，与硫酸链霉素相当。此外， 3还显着改变了青枯菌的形态并抑制了琥珀酸脱氢酶（SDH）的活性，干扰了青枯菌的生长。进行分子对接以阐明化合物3与SDH的潜在机制。进一步的体内实验表明， 3能显着抑制番茄青枯病的发生。

更新日期：2024-01-12

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