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Direct and robust citramalate production from brown macroalgae using fast-growing Vibrio sp. dhg
Bioresource Technology ( IF 9.7 ) Pub Date : 2024-01-10 , DOI: 10.1016/j.biortech.2024.130304
Hye Kyung Lee 1 , Sunghwa Woo 2 , Dongyeop Baek 2 , Myeongwon Min 1 , Gyoo Yeol Jung 3 , Hyun Gyu Lim 4
Affiliation  

Brown macroalgae is a promising feedstock for biorefinery owing to its high biomass productivity and contents of carbohydrates such as alginate and mannitol. However, the limited availability of microbial platforms efficiently catabolizing the brown macroalgae sugars has restricted its utilization. In this study, the direct production of citramalate, an important industrial compound, was demonstrated from brown macroalgae by utilizing Vibrio sp. dhg, which has a remarkably efficient catabolism of alginate and mannitol. Specifically, citramalate synthase from Methanocaldococcus jannaschii was synthetically expressed, and competing pathways were removed to maximally redirect the carbon flux toward citramalate production. Notably, a resulting strain, VXHC, produced citramalate up to 9.8 g/L from a 20 g/L mixture of alginate and mannitol regardless of their ratios. Citramalate was robustly produced even when diverse brown macroalgae were provided directly. Collectively, this study showcased the high potential of brown macroalgae biorefinery using Vibrio sp. dhg.



中文翻译:


使用快速生长的弧菌从棕色大型藻类中直接、稳定地生产柠檬酸。 DHG



褐藻由于其高生物质生产率和藻酸盐和甘露醇等碳水化合物的含量,是一种有前途的生物炼制原料。然而,有效分解巨藻糖的微生物平台的可用性有限,限制了其利用。在这项研究中,利用弧菌从棕色大型藻类中直接生产柠檬苹果酸(一种重要的工业化合物)。 DHG,对藻酸盐和甘露醇具有非常有效的分解代谢作用。具体而言,来自詹氏甲烷球菌的柠檬酸合酶被合成表达,并去除竞争途径以最大程度地将碳通量重定向至柠檬酸生产。值得注意的是,所得菌株 VXHC 从 20 g/L 海藻酸盐和甘露醇的混合物中产生高达 9.8 g/L 的柠檬酸,无论其比例如何。即使直接提供多种棕色大型藻类,柠檬苹果酸也能大量产生。总的来说,这项研究展示了使用弧菌进行棕色大型藻类生物精炼的巨大潜力。 DHG。

更新日期:2024-01-13
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